JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB79799

Recombinant human AKT3 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human AKT3 protein (His tag N-Terminus) is a Human Full Length protein, in the 2 to 479 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PKBG, AKT3, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, RAC-PK-gamma, STK-2, PKB gamma

2 Images
Functional Studies - Recombinant human AKT3 protein (His tag N-Terminus) (AB79799)
  • FuncS

Unknown

Functional Studies - Recombinant human AKT3 protein (His tag N-Terminus) (AB79799)

Kinase Assay : Specific Activity 10 pmol/min/μg.

SDS-PAGE - Recombinant human AKT3 protein (His tag N-Terminus) (AB79799)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human AKT3 protein (His tag N-Terminus) (AB79799)

10% SDS-PAGE, Coomassie staining.
Lane 1 : ab79799 5μg.
Lane 2 : Protein marker.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 14 pmol/min/μg.

Accession

Q9Y243

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB101133

Recombinant Human SIRT5 protein

SDS-PAGE - Recombinant Human SIRT5 protein (AB101133)

  • 0
  • 0
Proteins & Peptides

AB79798

Recombinant human AKT2 protein (His tag N-Terminus)

Functional Studies - Recombinant human AKT2 protein (His tag N-Terminus) (AB79798)

  • 0
  • 0
Multiplex Kits

AB283852

PI3K/AKT signalling pathway panel

Recombinant

Immunocytochemistry - PI3K/AKT signalling pathway panel (AB283852)

  • 5
  • 1
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MSPIDPMGHHHHHHGRRRASVAAGILVPRGSPGLDGICSIEEFSDVTIVKEGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDNERRPHFPQFSYSASGRE","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":479,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y243","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKT3 also known as protein kinase B gamma plays an important role in various cellular processes. This serine/threonine kinase with a molecular mass of approximately 56 kDa functions as part of the AKT family which includes AKT1 and AKT2. AKT3 expresses highly in the brain tissues with significant expression also observed in the heart and kidneys. As an isoform of the AKT kinase it participates in signaling pathways essential for cell survival and metabolism.
Biological function summary

AKT3 participates in regulating cell proliferation and survival. It operates as part of the PI3K/AKT signaling complex a central hub for signaling growth factors. This pathway is critical for transmitting signals from the cell surface to the nucleus affecting transcription and cell cycle progression. AKT3 protein specifically contributes significant roles in neuronal development and function due to its high expression within the nervous system.

Pathways

AKT3 influences the PI3K/AKT and mTOR pathways. These pathways regulate cell growth metabolism and survival playing significant roles in normal cellular functions and cancer. AKT3 interacts closely with other signaling molecules including mTORC1 and mTORC2 complexes. While participating in these pathways AKT3 coordinates with the protein E17K which is pattern related with alterations leading to abnormal signaling and growth.

AKT3 is implicated in cancer and neurological disorders. Aberrations in its signaling can lead to tumorigenesis as seen in glioblastomas. Abnormal AKT3 activity associates with epilepsy and other neurodevelopmental disorders emphasizing its impact on neuronal functions. Connecting AKT3 with disorders the protein E17K mutation in AKT3 dysregulates normal kinase activity contributing to these pathologies.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

Post-translational modifications

Phosphorylation on Thr-305 and Ser-472 is required for full activity (PubMed:12162751, PubMed:9512493). Phosphorylation of the activation loop at Thr-305 by PDPK1/PDK1 is a prerequisite for full activation (PubMed:9512493). Phosphorylation at Ser-472 by mTORC2 in response to growth factors plays a key role in AKT1 activation by facilitating subsequent phosphorylation of the activation loop by PDPK1/PDK1 (By similarity).. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDPK1/PDK1.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
See full target information AKT3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com