Recombinant Human AL-1 protein (His tag) is a Human Fragment protein, in the 1 to 203 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M L H V E M L T L V F L V L W M C V F S Q D P G S K A V A D R Y A V Y W N S S N P R F Q R G D Y H I D V C I N D Y L D V F C P H Y E D S V P E D K T E R Y V L Y M V N F D G Y S A C D H T S K G F K R W E C N R P H S P N G P L K F S E K F Q L F T P F S L G F E F R P G R E Y F Y I S S A I P D N G R R S C L K L K V F V R P T N S C M K T I G V H D R V F D V N D K V E N S L E P A D D T V H E S A E P S R G E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Alternative names
EPLG7, LERK7, EFNA5, Ephrin-A5, AL-1, EPH-related receptor tyrosine kinase ligand 7, LERK-7
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Recombinant Human AL-1 protein (His tag) is a Human Fragment protein, in the 1 to 203 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P52803-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M L H V E M L T L V F L V L W M C V F S Q D P G S K A V A D R Y A V Y W N S S N P R F Q R G D Y H I D V C I N D Y L D V F C P H Y E D S V P E D K T E R Y V L Y M V N F D G Y S A C D H T S K G F K R W E C N R P H S P N G P L K F S E K F Q L F T P F S L G F E F R P G R E Y F Y I S S A I P D N G R R S C L K L K V F V R P T N S C M K T I G V H D R V F D V N D K V E N S L E P A D D T V H E S A E P S R G E N
- Accession
- P52803
- Protein length
- Fragment
- Predicted molecular weight
- 23 kDa
- Amino acids
- 1 to 203
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
- Sequence similarities
Belongs to the ephrin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AL-1 also known as Anxa1 is a protein that plays a role in immune response and inflammation. It weighs approximately 38 kDa and can be found inside various tissues especially in immune cells like neutrophils and macrophages. Researchers often use animal models particularly in mouse studies to examine AL-1 functions due to its significance in biological processes.
- Biological function summary
The protein acts as a modulator in both innate and adaptive immune systems helping in the resolution of inflammation. AL-1 may function as part of a complex in these processes interacting with receptors on the cell surface. This protein regulates actions including cell migration and apoptotic cell clearance directly impacting how the body responds to infection and inflammation.
- Pathways
AL-1 influences key immunological processes via the phospholipase A2-inhibitory pathway. It can interact with proteins like S100A11 playing an important role in feedback mechanisms that control inflammation levels. AL-1 also interfaces with the annexin pathway which is important for modulating cell membrane interactions during immune responses.
- Associated diseases and disorders
AL-1 is linked with inflammatory diseases such as rheumatoid arthritis and systemic lupus erythematosus. In these conditions researchers found that altered AL-1 expression affects the inflammation resolution process. Connections have been made to related proteins like integrins which can modulate AL-1 activity in immune responses highlighting its potential as a therapeutic target for managing chronic inflammation.
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SDS-PAGE - Recombinant Human AL-1 protein (His tag) (ab276222)
SDS-PAGE analysis of ab276222
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