AB87415

Recombinant human ALDH2 protein (Active) (Tag Free)

Bioactive
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(3 Publications)

Recombinant human ALDH2 protein (Active) (Tag Free) is a Human Full Length protein, in the 18 to 517 aa range, expressed in Escherichia coli, with >90%, < 1 EU/mg endotoxin level, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

ALDM, ALDH2, ALDH class 2, ALDH-E2, ALDHI

1 Images

SDS-PAGE - Recombinant human ALDH2 protein (Active) (Tag Free) (AB87415)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ALDH2 protein (Active) (Tag Free) (AB87415)

SDS-PAGE analysis of ab87415 (3 μg) under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/mg

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.

Accession

P05091

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab87415 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/aldh2-antibody-ab70917'>ab70917</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

MW confirmed by MALDI-TOF.

Concentration determined by Absorbance at 280 nm.

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Sequence info

[{"sequence":"MSAAATQAVPAPNQQPEVFCNQIFINNEWHDAVSRKTFPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDRTYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS","proteinLength":"Full Length","predictedMolecularWeight":"54.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":517,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P05091","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.

Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.

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Specifications

Form

Liquid

Additional notes

ab87415 is purified using conventional chromatography techniques.

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General info

Function

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Post-translational modifications

In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation (PubMed:38297121). Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1 (PubMed:38297121).

Subcellular localisation

Mitochondrion matrix

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.

See full target information ALDH2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

SLAS discovery : advancing life sciences R & D 29:100154 PubMed38521503

2024

Aldehyde Dehydrogenase 2 (ALDH2): A novel sorafenib target in hepatocellular carcinoma unraveled by the proteome-wide cellular thermal shift assay.

Applications

Unspecified application

Species

Unspecified reactive species

Inês C Ferreira,Estefania Torrejón,Bernardo Abecasis,Bruno M Alexandre,Ricardo A Gomes,Chris Verslype,Jos van Pelt,Ana Barbas,Daniel Simão,Tiago M Bandeiras,Alessio Bortoluzzi,Sofia P Rebelo

Cellular & molecular immunology 21:510-526 PubMed38472357

2024

Neutrophil ALDH2 is a new therapeutic target for the effective treatment of sepsis-induced ARDS.

Applications

Unspecified application

Species

Unspecified reactive species

Changchang Xu,Lin Zhang,Shaoyu Xu,Zichen Wang,Qi Han,Ying Lv,Xingfang Wang,Xiangxin Zhang,Qingju Zhang,Ying Zhang,Simeng He,Qiuhuan Yuan,Yuan Bian,Chuanbao Li,Jiali Wang,Feng Xu,Yihai Cao,Jiaojiao Pang,Yuguo Chen

PloS one 12:e0170937 PubMed28129349

2017

A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Yasgar,Steven A Titus,Yuhong Wang,Carina Danchik,Shyh-Ming Yang,Vasilis Vasiliou,Ajit Jadhav,David J Maloney,Anton Simeonov,Natalia J Martinez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human ALDH2 protein (Active) (Tag Free)

SDS-PAGE - Recombinant human ALDH2 protein (Active) (Tag Free) (AB87415)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Subcellular localisation

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise