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AB99429

Recombinant Human ALDH5A1/SSADH protein

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Recombinant Human ALDH5A1/SSADH protein is a Human Full Length protein, in the 48 to 535 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SSADH, ALDH5A1, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase

1 Images
SDS-PAGE - Recombinant Human ALDH5A1/SSADH protein (AB99429)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ALDH5A1/SSADH protein (AB99429)

SDS-PAGE analysis of ab99429 (3 μg) under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P51649

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ALDH5A1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAGRLAGLSAALLRTDSFVGGRWLPAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPFSALALAELASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPLVSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL","proteinLength":"Full Length","predictedMolecularWeight":"54.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":535,"aminoAcidStart":48,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P51649","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH5A1 also known as succinate semialdehyde dehydrogenase (SSADH) functions in the cellular metabolism where it converts succinate semialdehyde to succinate in the presence of NAD+. This enzyme has a molecular weight of approximately 56 kDa and is largely found in the brain as well as in other organs like liver and kidney. ALDH5A1 operates in mitochondria emphasizing its role in cellular respiration and energy production.
Biological function summary

The enzyme ALDH5A1 plays an important role in the breakdown of gamma-aminobutyric acid (GABA) an important neurotransmitter. It is known to be part of the GABA degradation complex which controls the levels of GABA in the central nervous system. Balance in GABA concentration is necessary for proper neurotransmission and neurophysiological functions. Consequently ALDH5A1 helps in maintaining the inhibitory effects GABA has on neurons.

Pathways

ALDH5A1 is significant in the GABA shunt pathway an alternative mechanism for glutamate and GABA metabolism. The enzyme collaborates closely with GABA transaminase which converts GABA into succinate semialdehyde before ALDH5A1 further processes it into succinate. This pathway supports energy production and the regulation of carbon flow through central metabolism.

ALDH5A1 deficiencies link to disorders like Succinic Semialdehyde Dehydrogenase deficiency (SSADH deficiency) and GABA-transaminase deficiency. These conditions result in the accumulation of gamma-hydroxybutyric acid leading to neurological complications. This enzyme interacts with proteins like GABA transaminase which shows the importance of its proper function in preventing neurologic disease.
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Specifications

Form

Liquid

Additional notes

ab99429 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Subcellular localisation

Mitochondrion

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
See full target information ALDH5A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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