Recombinant human Aldolase C protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
Amino acid sequence
M P H S Y P A L S A E Q K K E L S D I A L R I V A P G K G I L A A D E S V G S M A K R L S Q I G V E N T E E N R R L Y R Q V L F S A D D R V K K C I G G V I F F H E T L Y Q K D D N G V P F V R T I Q D K G I V V G I K V D K G V V P L A G T D G E T T T Q G L D G L S E R C A Q Y K K D G A D F A K W R C V L K I S E R T P S A L A I L E N A N V L A R Y A S I C Q Q N G I V P I V E P E I L P D G D H D L K R C Q Y V T E K V L A A V Y K A L S D H H V Y L E G T L L K P N M V T P G H A C P I K Y T P E E I A M A T V T A L R R T V P P A V P G V T F L S G G Q S E E E A S F N L N A I N R C P L P R P W A L T F S Y G R A L Q A S A L N A W R G Q R D N A G A A T E E F I K R A E V N G L A A Q G K Y E G S G E D G G A A A Q S L Y I A N H A Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Alternative names
ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase
Recommended products
Recombinant human Aldolase C protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 6 units/mg. One unit will convert 1.0 μmol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37°C.
- Accession
- P09972-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M P H S Y P A L S A E Q K K E L S D I A L R I V A P G K G I L A A D E S V G S M A K R L S Q I G V E N T E E N R R L Y R Q V L F S A D D R V K K C I G G V I F F H E T L Y Q K D D N G V P F V R T I Q D K G I V V G I K V D K G V V P L A G T D G E T T T Q G L D G L S E R C A Q Y K K D G A D F A K W R C V L K I S E R T P S A L A I L E N A N V L A R Y A S I C Q Q N G I V P I V E P E I L P D G D H D L K R C Q Y V T E K V L A A V Y K A L S D H H V Y L E G T L L K P N M V T P G H A C P I K Y T P E E I A M A T V T A L R R T V P P A V P G V T F L S G G Q S E E E A S F N L N A I N R C P L P R P W A L T F S Y G R A L Q A S A L N A W R G Q R D N A G A A T E E F I K R A E V N G L A A Q G K Y E G S G E D G G A A A Q S L Y I A N H A Y
- Accession
- P09972
- Protein length
- Full Length
- Predicted molecular weight
- 39.4 kDa
- Amino acids
- 1 to 364
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab227413 was purified using conventional chromatography techniques.
General info
- Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldolase C also known as ALDOC is an enzyme that plays an important role in glycolysis. It catalyzes the reversible conversion of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Aldolase C has a molecular mass of approximately 39 kDa. This enzyme is primarily expressed in the brain particularly in astrocytes where it serves as an identifier for these cells.
- Biological function summary
This enzyme contributes to energy metabolism within cells notably astrocytes. It is part of the aldolase family which includes isoenzymes like aldolase A and B that perform similar functions in different tissues. Aldolase C is involved in maintaining glucose homeostasis and energy production in the brain where it forms part of a complex with other glycolytic enzymes to facilitate efficient cellular respiration.
- Pathways
Aldolase C is a significant component of the glycolytic pathway facilitating energy production in cells. It plays alongside other glycolytic enzymes such as hexokinase phosphofructokinase and pyruvate kinase to convert glucose into pyruvate transferring energy in the process. Additionally aldolase C participates in the fructose metabolism pathway similar to its isoenzymes aiding in the conversion of fructose derivatives important for maintaining normal energy levels in brain tissues.
- Associated diseases and disorders
Aldolase C has connections to neurological conditions like glioma and neurodegenerative diseases. Altered expression levels of aldolase C can indicate glioma progression or serve as a biomarker in astrocytic neurodegenerative disorders. The enzyme’s function is also linked to its interaction with other proteins such as Glial Fibrillary Acidic Protein (GFAP) in astrocytes which assist in maintaining normal cell structure and response to damage. Therefore aldolase C serves both as a metabolic enzyme and a potential marker in assessing neurological health and disease.
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1 product image
SDS-PAGE - Recombinant human Aldolase C protein (ab227413)
15% SDS-PAGE analysis of 3 μg ab227413.
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