Recombinant Human ALDR1 Protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
M A S R L L L N N G A K M P I L G L G T W K S P P G Q V T E A V K V A I D V G Y R H I D C A H V Y Q N E N E V G V A I Q E K L R E Q V V K R E E L F I V S K L W C T Y H E K G L V K G A C Q K T L S D L K L D Y L D L Y L I H W P T G F K P G K E F F P L D E S G N V V P S D T N I L D T W A A M E E L V D E G L V K A I G I S N F N H L Q V E M I L N K P G L K Y K P A V N Q I E C H P Y L T Q E K L I Q Y C Q S K G I V V T A Y S P L G S P D R P W A K P E D P S L L E D P R I K A I A A K H N K T T A Q V L I R F P M Q R N L V V I P K S V T P E R I A E N F K V F D F E L S S Q D M T T L L S Y N R N W R V C A L L S C T S H K D Y P F H E E F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).
Alternative names
ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR
Recommended products
Recombinant Human ALDR1 Protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P15121-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.3002% Tris HCl, 0.0297% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A S R L L L N N G A K M P I L G L G T W K S P P G Q V T E A V K V A I D V G Y R H I D C A H V Y Q N E N E V G V A I Q E K L R E Q V V K R E E L F I V S K L W C T Y H E K G L V K G A C Q K T L S D L K L D Y L D L Y L I H W P T G F K P G K E F F P L D E S G N V V P S D T N I L D T W A A M E E L V D E G L V K A I G I S N F N H L Q V E M I L N K P G L K Y K P A V N Q I E C H P Y L T Q E K L I Q Y C Q S K G I V V T A Y S P L G S P D R P W A K P E D P S L L E D P R I K A I A A K H N K T T A Q V L I R F P M Q R N L V V I P K S V T P E R I A E N F K V F D F E L S S Q D M T T L L S Y N R N W R V C A L L S C T S H K D Y P F H E E F
- Accession
- P15121
- Protein length
- Full Length
- Predicted molecular weight
- 35.91 kDa
- Actual molecular weight
- 35.91 kDa
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).
- Sequence similarities
Belongs to the aldo/keto reductase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.
- Biological function summary
The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.
- Pathways
Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.
- Associated diseases and disorders
Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.
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2 product images
SDS-PAGE - Recombinant Human ALDR1 Protein (ab318943)
SDS-PAGE analysis of ab318943 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Human ALDR1 Protein (ab318943)
Mass determination by ESI-TOF.
Predicted MW is 35910 Da (+/- 10 Da by ESI-TOF). Observed MW is 35917.27 Da.
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