Recombinant Human ALIX Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17556548, PubMed:17853893). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17556548, PubMed:17853893, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity). (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
Alternative names
AIP1, ALIX, KIAA1375, PDCD6IP, Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95
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Recombinant Human ALIX Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
- Accession
- Q8WUM4-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- Q8WUM4
- Protein length
- Fragment
- Predicted molecular weight
- 98.3 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17556548, PubMed:17853893). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17556548, PubMed:17853893, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).
- Post-translational modifications
May be phosphorylated on tyrosine residues by activated PDGFRB.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
- Biological function summary
ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.
- Pathways
ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.
- Associated diseases and disorders
ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.
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2 product images
Sandwich ELISA - Recombinant Human ALIX Protein Standard (His tag) (ab322103)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
SDS-PAGE - Recombinant Human ALIX Protein Standard (His tag) (ab322103)
SDS-PAGE analysis of ab322103 under reducing conditions for 2ug protein.
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