Recombinant Human Alkaline Phosphatase protein is a Human Full Length protein, in the 1 to 532 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M Q G P W V L L L L G L R L Q L S L G I I P V E E E N P D F W N R Q A A E A L G A A K K L Q P A Q T A A K N L I I F L G D G M G V S T V T A A R I L K G Q K K D K L G P E T F L A M D R F P Y V A L S K T Y S V D K H V P D S G A T A T A Y L C G V K G N F Q T I G L S A A A R F N Q C N T T R G N E V I S V M N R A K K A G K S V G V V T T T R V Q H A S P A G A Y A H T V N R N W Y S D A D V P A S A R Q E G C Q D I A T Q L I S N M D I D V I L G G G R K Y M F P M G T P D P E Y P D D Y S Q G G T R L D G K N L V Q E W L A K R Q G A R Y V W N R T E L M Q A S L D P S V T H L M G L F E P G D M K Y E I H R D S T L D P S L M E M T E A A L R L L S R N P R G F F L F V E G G R I D H G H H E S R A Y R A L T E T I M F D D A I E R A G Q L T S E E D T L S L V T A D H S H V F S F G G Y P L R G S S I F G L A P G K A R D R K A Y T V L L Y G N G P G Y V L K D G A R P D V T E S E S G S P E Y R Q Q S A V P L D G E T H A G E D V A V F A R G P Q A H L V H G V Q E Q T F I A H V M A F A A C L E P Y T A C D L A P R A G T T D A A H P G P S V V P A L L P L L A G T L L L L G T A T A P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Alternative names
Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI
Recommended products
Recombinant Human Alkaline Phosphatase protein is a Human Full Length protein, in the 1 to 532 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P09923-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M Q G P W V L L L L G L R L Q L S L G I I P V E E E N P D F W N R Q A A E A L G A A K K L Q P A Q T A A K N L I I F L G D G M G V S T V T A A R I L K G Q K K D K L G P E T F L A M D R F P Y V A L S K T Y S V D K H V P D S G A T A T A Y L C G V K G N F Q T I G L S A A A R F N Q C N T T R G N E V I S V M N R A K K A G K S V G V V T T T R V Q H A S P A G A Y A H T V N R N W Y S D A D V P A S A R Q E G C Q D I A T Q L I S N M D I D V I L G G G R K Y M F P M G T P D P E Y P D D Y S Q G G T R L D G K N L V Q E W L A K R Q G A R Y V W N R T E L M Q A S L D P S V T H L M G L F E P G D M K Y E I H R D S T L D P S L M E M T E A A L R L L S R N P R G F F L F V E G G R I D H G H H E S R A Y R A L T E T I M F D D A I E R A G Q L T S E E D T L S L V T A D H S H V F S F G G Y P L R G S S I F G L A P G K A R D R K A Y T V L L Y G N G P G Y V L K D G A R P D V T E S E S G S P E Y R Q Q S A V P L D G E T H A G E D V A V F A R G P Q A H L V H G V Q E Q T F I A H V M A F A A C L E P Y T A C D L A P R A G T T D A A H P G P S V V P A L L P L L A G T L L L L G T A T A P
- Accession
- P09923
- Protein length
- Full Length
- Amino acids
- 1 to 532
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
- Sequence similarities
Belongs to the alkaline phosphatase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.
- Biological function summary
Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.
- Pathways
Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.
- Associated diseases and disorders
Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.
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1 product image
SDS-PAGE - Recombinant Human Alkaline Phosphatase protein (ab157880)
ab157880 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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