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AB132191

Recombinant Human ALKBH1 protein (GST tag N-Terminus)

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Recombinant Human ALKBH1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 389 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ABH, ABH1, ALKBH, ALKBH1, Nucleic acid dioxygenase ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, mRNA N(3)-methylcytidine demethylase

1 Images
SDS-PAGE - Recombinant Human ALKBH1 protein (GST tag N-Terminus) (AB132191)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ALKBH1 protein (GST tag N-Terminus) (AB132191)

12.5% SDS-PAGE analysis of ab132191 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Q13686

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB195376

Anti-ALKBH1 antibody [EPR19215]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (AB195376)

  • 5
  • 1
Primary Antibodies

AB208198

Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]

RabMAb|Recombinant|Trial Size

ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

  • 0
  • 0
Primary Antibodies

AB126605

Anti-FTO antibody [EPR6894]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6894] (AB126605)

  • 4
  • 3
Primary Antibodies

AB195377

Anti-ALKBH5 antibody [EPR18958]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH5 antibody [EPR18958] (AB195377)

  • 4
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDLGFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLPNPEGEGLPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQVLATDQNFPLEPIEDEKRDISTEGFCHLDDQNSEVKRARINPHS","proteinLength":"Full Length","predictedMolecularWeight":"68.42 kDa","actualMolecularWeight":null,"aminoAcidEnd":389,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q13686","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Dioxygenase that acts on nucleic acids, such as DNA and tRNA (PubMed : 18603530, PubMed : 27497299, PubMed : 27745969). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 18603530, PubMed : 27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PubMed : 27497299, PubMed : 27745969). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed : 27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation : regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed : 27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (PubMed : 27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed : 27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA (PubMed : 30017583, PubMed : 30392959). N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity). Demethylates mRNAs containing N(3)-methylcytidine modification (PubMed : 31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PubMed : 18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites : cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed : 19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed : 19959401, PubMed : 23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity).

Sequence similarities

Belongs to the alkB family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Dioxygenase that acts on nucleic acids, such as DNA and tRNA (PubMed : 18603530, PubMed : 27497299, PubMed : 27745969). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 18603530, PubMed : 27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PubMed : 27497299, PubMed : 27745969). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed : 27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation : regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed : 27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (PubMed : 27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed : 27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA (PubMed : 30017583, PubMed : 30392959). N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity). Demethylates mRNAs containing N(3)-methylcytidine modification (PubMed : 31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PubMed : 18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites : cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed : 19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed : 19959401, PubMed : 23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity).
See full target information ALKBH1
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