Recombinant Human ALKBH2 protein

Specifications

Form

Liquid

Additional notes

ab105622 was purified using conventional chromatography techniques.

General info

Function

Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 20714506, PubMed : 23972994, PubMed : 25797601). Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis (PubMed : 18432238, PubMed : 22659876). Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 20714506, PubMed : 23972994, PubMed : 25797601, PubMed : 26408825). Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription (PubMed : 23972994). In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication (PubMed : 19736315, PubMed : 26408825).

Sequence similarities

Belongs to the alkB family.

Subcellular localisation

Nucleus