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Recombinant Human ALOX12B protein is a Human Full Length protein, in the 1 to 701 aa range, expressed in Wheat germ and suitable for ELISA, WB.

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Images

SDS-PAGE - Recombinant Human ALOX12B protein (AB157877), expandable thumbnail

Key facts

Expression system
Wheat germ
Tags
GST tag N-Terminus
Applications
ELISA, WB
Biologically active
No

Amino acid sequence

M A T Y K V R V A T G T D L L S G T R D S I S L T I V G T Q G E S H K Q L L N H F G R D F A T G A V G Q Y T V Q C P Q D L G E L I I I R L H K E R Y A F F P K D P W Y C N Y V Q I C A P N G R I Y H F P A Y Q W M D G Y E T L A L R E A T G K T T A D D S L P V L L E H R K E E I R A K Q D F Y H W R V F L P G L P S Y V H I P S Y R P P V R R H R N P N R P E W N G Y I P G F P I L I N F K A T K F L N L N L R Y S F L K T A S F F V R L G P M A L A F K V R G L L D C K H S W K R L K D I R K I F P G K K S V V S E Y V A E H W A E D T F F G Y Q Y L N G V N P G L I R R C T R I P D K F P V T D D M V A P F L G E G T C L Q A E L E K G N I Y L A D Y R I M E G I P T V E L S G R K Q H H C A P L C L L H F G P E G K M M P I A I Q L S Q T P G P D C P I F L P S D S E W D W L L A K T W V R Y A E F Y S H E A I A H L L E T H L I A E A F C L A L L R N L P M C H P L Y K L L I P H T R Y T V Q I N S I G R A V L L N E G G L S A K G M S L G V E G F A G V M V R A L S E L T Y D S L Y L P N D F V E R G V Q D L P G Y Y Y R D D S L A V W N A L E K Y V T E I I T Y Y Y P S D A A V E G D P E L Q S W V Q E I F K E C L L G R E S S G F P R C L R T V P E L I R Y V T I V I Y T C S A K H A A V N T G Q M E F T A W M P N F P A S M R N P P I Q T K G L T T L E T F M D T L P D V K T T C I T L L V L W T L S R E P D D R R P L G H F P D I H F V E E A P R R S I E A F R Q R L N Q I S H D I R Q R N K C L P I P Y Y Y L D P V L I E N S I S I

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:21558561, PubMed:9618483, PubMed:9837935). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738).

Alternative names

Recommended products

Recombinant Human ALOX12B protein is a Human Full Length protein, in the 1 to 701 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Key facts

Expression system
Wheat germ
Applications
ELISA, WB
Accession
O75342-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M A T Y K V R V A T G T D L L S G T R D S I S L T I V G T Q G E S H K Q L L N H F G R D F A T G A V G Q Y T V Q C P Q D L G E L I I I R L H K E R Y A F F P K D P W Y C N Y V Q I C A P N G R I Y H F P A Y Q W M D G Y E T L A L R E A T G K T T A D D S L P V L L E H R K E E I R A K Q D F Y H W R V F L P G L P S Y V H I P S Y R P P V R R H R N P N R P E W N G Y I P G F P I L I N F K A T K F L N L N L R Y S F L K T A S F F V R L G P M A L A F K V R G L L D C K H S W K R L K D I R K I F P G K K S V V S E Y V A E H W A E D T F F G Y Q Y L N G V N P G L I R R C T R I P D K F P V T D D M V A P F L G E G T C L Q A E L E K G N I Y L A D Y R I M E G I P T V E L S G R K Q H H C A P L C L L H F G P E G K M M P I A I Q L S Q T P G P D C P I F L P S D S E W D W L L A K T W V R Y A E F Y S H E A I A H L L E T H L I A E A F C L A L L R N L P M C H P L Y K L L I P H T R Y T V Q I N S I G R A V L L N E G G L S A K G M S L G V E G F A G V M V R A L S E L T Y D S L Y L P N D F V E R G V Q D L P G Y Y Y R D D S L A V W N A L E K Y V T E I I T Y Y Y P S D A A V E G D P E L Q S W V Q E I F K E C L L G R E S S G F P R C L R T V P E L I R Y V T I V I Y T C S A K H A A V N T G Q M E F T A W M P N F P A S M R N P P I Q T K G L T T L E T F M D T L P D V K T T C I T L L V L W T L S R E P D D R R P L G H F P D I H F V E E A P R R S I E A F R Q R L N Q I S H D I R Q R N K C L P I P Y Y Y L D P V L I E N S I S I
Accession
O75342
Protein length
Full Length
Amino acids
1 to 701
Nature
Recombinant
Tags
GST tag N-Terminus

Specifications

Form
Liquid

General info

Function

Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:21558561, PubMed:9618483, PubMed:9837935). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738).

Sequence similarities

Belongs to the lipoxygenase family.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

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1 product image

  • SDS-PAGE - Recombinant Human ALOX12B protein (ab157877), expandable thumbnail

    SDS-PAGE - Recombinant Human ALOX12B protein (ab157877)

    ab157877 on a 12.5% SDS-PAGE stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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