Recombinant human alpha 1 Antitrypsin protein (Active) is a Human Full Length protein, in the 25 to 418 aa range with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry and HPLC. The predicted molecular weight of ab282404 protein is 44.4 kDa.
- Save time and ensure accurate results - use our recombinant alpha 1 Antitrypsin/Serpin A1 protein
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
E D P Q G D A A Q K T D T S H H D Q D H P T F N K I T P N L A E F A F S L Y R Q L A H Q S N S T N I F F S P V S I A T A F A M L S L G T K A D T H D E I L E G L N F N L T E I P E A Q I H E G F Q E L L R T L N Q P D S Q L Q L T T G N G L F L S E G L K L V D K F L E D V K K L Y H S E A F T V N F G D T E E A K K Q I N D Y V E K G T Q G K I V D L V K E L D R D T V F A L V N Y I F F K G K W E R P F E V K D T E E E D F H V D Q V T T V K V P M M K R L G M F N I Q H C K K L S S W V L L M K Y L G N A T A I F F L P D E G K L Q H L E N E L T H D I I T K F L E N E D R R S A S L H L P K L S I T G T Y D L K S V L G Q L G I T K V F S N G A D L S G V T E E A P L K L S K A V H K A V L T I D E K G T E A A G A M F L E A I P M S I P P E V K F N K P F V F L M I E Q N T K S P L F M G K V V N P T Q K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Alternative names
AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1
Recommended products
Recombinant human alpha 1 Antitrypsin protein (Active) is a Human Full Length protein, in the 25 to 418 aa range with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry and HPLC. The predicted molecular weight of ab282404 protein is 44.4 kDa.
- Save time and ensure accurate results - use our recombinant alpha 1 Antitrypsin/Serpin A1 protein
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully active measured by its ability to inhibit trypsin cleavage of fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.
ED50 for this effect is ≤18 ng/ml corresponding to a specific activity of 5.55 x 104 units/mg.
- Accession
- P01009-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- E D P Q G D A A Q K T D T S H H D Q D H P T F N K I T P N L A E F A F S L Y R Q L A H Q S N S T N I F F S P V S I A T A F A M L S L G T K A D T H D E I L E G L N F N L T E I P E A Q I H E G F Q E L L R T L N Q P D S Q L Q L T T G N G L F L S E G L K L V D K F L E D V K K L Y H S E A F T V N F G D T E E A K K Q I N D Y V E K G T Q G K I V D L V K E L D R D T V F A L V N Y I F F K G K W E R P F E V K D T E E E D F H V D Q V T T V K V P M M K R L G M F N I Q H C K K L S S W V L L M K Y L G N A T A I F F L P D E G K L Q H L E N E L T H D I I T K F L E N E D R R S A S L H L P K L S I T G T Y D L K S V L G Q L G I T K V F S N G A D L S G V T E E A P L K L S K A V H K A V L T I D E K G T E A A G A M F L E A I P M S I P P E V K F N K P F V F L M I E Q N T K S P L F M G K V V N P T Q K
- Accession
- P01009
- Protein length
- Full Length
- Predicted molecular weight
- 44.38 kDa
- Amino acids
- 25 to 418
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
- Sequence similarities
Belongs to the serpin family.
- Post-translational modifications
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
- Biological function summary
A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.
- Pathways
Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.
- Associated diseases and disorders
Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
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4 product images
Functional Studies - Recombinant human alpha 1 Antitrypsin protein (Active) (ab282404)
Fully active measured by its ability to inhibit trypsin cleavage of fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.
ED50 for this effect is ≤18 ng/ml corresponding to a specific activity of 5.55 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3396379-2
SDS-PAGE - Recombinant human alpha 1 Antitrypsin protein (Active) (ab282404)
SDS-page analysis of ab282404.
Mass Spectrometry - Recombinant human alpha 1 Antitrypsin protein (Active) (ab282404)
Mass determination by ESI-TOF.
Predicted MW is 44381.60 Da (+/- 10 Da by ESI-TOF). Observed MW is 44386.58
HPLC - Recombinant human alpha 1 Antitrypsin protein (Active) (ab282404)
HPLC analysis of ab282404.
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