Recombinant Human alpha 1 Fetoprotein is a Human Full Length protein, in the 1 to 609 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Publications
- Scientific reports 6:311322016A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiying Zhang et. al.PubMed 27528397
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M K W V E S I F L I F L L N F T E S R T L H R N E Y G I A S I L D S Y Q C T A E I S L A D L A T I F F A Q F V Q E A T Y K E V S K M V K D A L T A I E K P T G D E Q S S G C L E N Q L P A F L E E L C H E K E I L E K Y G H S D C C S Q S E E G R H N C F L A H K K P T P A S I P L F Q V P E P V T S C E A Y E E D R E T F M N K F I Y E I A R R H P F L Y A P T I L L W A A R Y D K I I P S C C K A E N A V E C F Q T K A A T V T K E L R E S S L L N Q H A C A V M K N F G T R T F Q A I T V T K L S Q K F T K V N F T E I Q K L V L D V A H V H E H C C R G D V L D C L Q D G E K I M S Y I C S Q Q D T L S N K I T E C C K L T T L E R G Q C I I H A E N D E K P E G L S P N L N R F L G D R D F N Q F S S G E K N I F L A S F V H E Y S R R H P Q L A V S V I L R V A K G Y Q E L L E K C F Q T E N P L E C Q D K G E E E L Q K Y I Q E S Q A L A K R S C G L F Q K L G E Y Y L Q N A F L V A Y T K K A P Q L T S S E L M A I T R K M A A T A A T C C Q L S E D K L L A C G E G A A D I I I G H L C I R H E M T P V N T G V G Q C C T S S Y A N R R P C F S S L V V D E T Y V P P A F S D D K F I F H K D L C Q A Q G V A L Q T M K Q E F L I N L V K Q K P Q I T E E Q L E A V I A D F S G L L E K C C Q G Q E Q E V C F A E E G Q K L I S K T R A A L G V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Alternative names
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP, AFP
Recommended products
Recombinant Human alpha 1 Fetoprotein is a Human Full Length protein, in the 1 to 609 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP, AFP
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P02771-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
M K W V E S I F L I F L L N F T E S R T L H R N E Y G I A S I L D S Y Q C T A E I S L A D L A T I F F A Q F V Q E A T Y K E V S K M V K D A L T A I E K P T G D E Q S S G C L E N Q L P A F L E E L C H E K E I L E K Y G H S D C C S Q S E E G R H N C F L A H K K P T P A S I P L F Q V P E P V T S C E A Y E E D R E T F M N K F I Y E I A R R H P F L Y A P T I L L W A A R Y D K I I P S C C K A E N A V E C F Q T K A A T V T K E L R E S S L L N Q H A C A V M K N F G T R T F Q A I T V T K L S Q K F T K V N F T E I Q K L V L D V A H V H E H C C R G D V L D C L Q D G E K I M S Y I C S Q Q D T L S N K I T E C C K L T T L E R G Q C I I H A E N D E K P E G L S P N L N R F L G D R D F N Q F S S G E K N I F L A S F V H E Y S R R H P Q L A V S V I L R V A K G Y Q E L L E K C F Q T E N P L E C Q D K G E E E L Q K Y I Q E S Q A L A K R S C G L F Q K L G E Y Y L Q N A F L V A Y T K K A P Q L T S S E L M A I T R K M A A T A A T C C Q L S E D K L L A C G E G A A D I I I G H L C I R H E M T P V N T G V G Q C C T S S Y A N R R P C F S S L V V D E T Y V P P A F S D D K F I F H K D L C Q A Q G V A L Q T M K Q E F L I N L V K Q K P Q I T E E Q L E A V I A D F S G L L E K C C Q G Q E Q E V C F A E E G Q K L I S K T R A A L G V
- Accession
- Protein length
Full Length
- Predicted molecular weight
95 kDa
- Amino acids
1 to 609
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
- Sequence similarities
Belongs to the ALB/AFP/VDB family.
- Post-translational modifications
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Protein concentration is lot specific and is above or equal to 0.05 mg/mL.
Supplementary info
- Activity summary
Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
- Biological function summary
Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.
- Pathways
Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.
- Associated diseases and disorders
High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
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1 product image
SDS-PAGE - Recombinant Human alpha 1 Fetoprotein (ab114216)
ab114216 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
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