Recombinant Human alpha Actinin 4 protein is a Human Fragment protein, in the 397 to 648 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).
Alpha-actinin-4, Non-muscle alpha-actinin 4, ACTN4
Recombinant Human alpha Actinin 4 protein is a Human Fragment protein, in the 397 to 648 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
Constituents: 0.58% Sodium chloride, 0.32% Tris HCl
Purified via His tag
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882).
Belongs to the alpha-actinin family.
Alpha Actinin 4 sometimes referred to as ACTN4 or alpha-actinin is a protein with a mass of approximately 100 kDa. It functions as an actin-binding protein particularly involved in the crosslinking of actin filaments. This protein is expressed widely with high levels found in muscle kidney and brain tissues. Alpha-actinin 4 also plays important roles in cellular structure and integrity by binding actin filaments in various cellular contexts.
The role of alpha-actinin 4 extends to maintaining cytoskeletal structure and cell motility. It forms part of the larger actin-binding complex that supports cellular adhesion and mechanical stability. By linking actin filaments it allows cells to withstand mechanical stress and maintain their shape. Additionally alpha-actinin 4 contributes to cellular processes like cytokinesis and signal transduction by serving as a scaffold for signaling proteins.
Alpha-actinin 4 interacts with diverse cellular signaling mechanisms. Key pathways include the actin cytoskeleton signaling and integrin-mediated cell adhesion. In these pathways alpha-actinin 4 works alongside proteins such as integrins and vinculin facilitating communication between the extracellular matrix and the cytoskeleton. These interactions are critical for cellular responses to environmental stimuli and adaptation during movement or development.
Alpha-actinin 4 has notable impacts on conditions like focal segmental glomerulosclerosis (FSGS) and cancer. In FSGS mutation or dysregulation of alpha-actinin 4 contributes to podocyte dysfunction leading to proteinuria and kidney damage. Its role in cancer often involves altered expression influencing cell migration and invasion. The interaction with other proteins like nephrin in FSGS or E-cadherin in cancer highlights the significance of alpha-actinin 4 in pathogenic processes.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com