Recombinant Human alpha smooth muscle Actin protein is a Human Full Length protein, in the 3 to 377 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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- eLife 7:2018α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Gordon et. al.PubMed 30260317
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M E E E D S T A L V C D N G S G L C K A G F A G D D A P R A V F P S I V G R P R H Q G V M V G M G Q K D S Y V G D E A Q S K R G I L T L K Y P I E H G I I T N W D D M E K I W H H S F Y N E L R V A P E E H P T L L T E A P L N P K A N R E K M T Q I M F E T F N V P A M Y V A I Q A V L S L Y A S G R T T G I V L D S G D G V T H N V P I Y E G Y A L P H A I M R L D L A G R D L T D Y L M K I L T E R G Y S F V T T A E R E I V R D I K E K L C Y V A L D F E N E M A T A A S S S S L E K S Y E L P D G Q V I T I G N E R F R C P E T L F Q P S F I G M E S A G I H E T T Y N S I M K C D I D I R K D L Y A N N V L S G G T T M Y P G I A D R M Q K E I T A L A P S T M K I K I I A P P E R K Y S V W I G G S I L A S L S T F Q Q M W I S K Q E Y D E A G P S I V H R K C F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
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Recombinant Human alpha smooth muscle Actin protein is a Human Full Length protein, in the 3 to 377 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P62736-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M E E E D S T A L V C D N G S G L C K A G F A G D D A P R A V F P S I V G R P R H Q G V M V G M G Q K D S Y V G D E A Q S K R G I L T L K Y P I E H G I I T N W D D M E K I W H H S F Y N E L R V A P E E H P T L L T E A P L N P K A N R E K M T Q I M F E T F N V P A M Y V A I Q A V L S L Y A S G R T T G I V L D S G D G V T H N V P I Y E G Y A L P H A I M R L D L A G R D L T D Y L M K I L T E R G Y S F V T T A E R E I V R D I K E K L C Y V A L D F E N E M A T A A S S S S L E K S Y E L P D G Q V I T I G N E R F R C P E T L F Q P S F I G M E S A G I H E T T Y N S I M K C D I D I R K D L Y A N N V L S G G T T M Y P G I A D R M Q K E I T A L A P S T M K I K I I A P P E R K Y S V W I G G S I L A S L S T F Q Q M W I S K Q E Y D E A G P S I V H R K C F
- Accession
- P62736
- Protein length
- Full Length
- Predicted molecular weight
- 44.4 kDa
- Amino acids
- 3 to 377
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134555 is purified using conventional chromatography techniques.
General info
- Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Sequence similarities
Belongs to the actin family.
- Post-translational modifications
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
- Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
- Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
- Associated diseases and disorders
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
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SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (ab134555)
15% SDS-PAGE analysis of 3 µg ab134555.
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