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AB100963

Recombinant Human Alpha SNAP protein

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Recombinant Human Alpha SNAP protein is a Human Full Length protein, in the 1 to 295 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SNAPA, NAPA, Alpha-soluble NSF attachment protein, SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha

1 Images
SDS-PAGE - Recombinant Human Alpha SNAP protein (AB100963)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Alpha SNAP protein (AB100963)

15% SDS-PAGE showing ab100963 (3 μg) at approximately 35.3 kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P54920

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR","proteinLength":"Full Length","predictedMolecularWeight":"35.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":295,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P54920","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha SNAP also known as NSF attachment protein alpha is an important component of cellular machinery. This protein weighs approximately 33 kDa and facilitates membrane trafficking processes. It is expressed ubiquitously meaning it is found throughout various tissues in the body. Alpha SNAP is essential for the proper function of membrane fusion events playing a significant role in exocytosis and endocytosis.
Biological function summary

Alpha SNAP works as a part of the SNARE complex. It acts as a mediator that helps recycle SNARE proteins which are key components in vesicle fusion processes. The protein binds to the SNARE complex allowing NSF (N-ethylmaleimide-sensitive factor) to disassemble it enabling the components to be reused. This recycling is critical for continuous vesicular transport aiding in the maintenance of cellular function.

Pathways

Alpha SNAP functions mainly in the vesicular transport and membrane trafficking pathways. It interacts with NSF and other SNARE proteins to facilitate these processes. Within the cellular transport pathway Alpha SNAP ensures efficient neurotransmitter release and hormone secretion. It is involved in the endocytic and exocytic pathways both vital for proper cellular communication and function.

Mutations or dysfunctions in Alpha SNAP have been linked to neurological disorders such as epilepsy due to its role in neurotransmitter release. Its malfunction can disrupt synaptic communication leading to abnormal neural activity. Additionally Alpha SNAP may be implicated in some types of neurodegenerative diseases interacting with proteins such as NSF which are important for synaptic maintenance and plasticity.
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Specifications

Form

Liquid

Additional notes

ab100963 is purified using conventional chromatography techniques.

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General info

Function

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed : 15980433).

Sequence similarities

Belongs to the SNAP family.

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Product protocols

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Target data

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed : 15980433).
See full target information NAPA
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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB133673

Anti-Alpha SNAP antibody [EPR7372]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (AB133673)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
  • 0

Product promise

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