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Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (AB256149), expandable thumbnail
  • SDS-PAGE - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (AB256149), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS
Biological activity
100 μM ab256149 seeded with 10 nM ab256150 in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Accession
P37840-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: PBS

Sequence info

Amino acid sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V T T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
Accession
P37840
Protein length
Full Length
Predicted molecular weight
14.46 kDa
Amino acids
1 to 140
Nature
Recombinant

Specifications

Form
Liquid
Additional notes

Purified by ion-exchange chromatography.

General info

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity.

This supplementary information is collated from multiple sources and compiled automatically.

Sequence similarities

Belongs to the synuclein family.

Post-translational modifications

Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Monomer.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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2 product images

  • Functional Studies - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (ab256149), expandable thumbnail

    Functional Studies - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (ab256149)

    Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (ab256149). A much greater increase in fluorescence is seen when 100 μM monomer (ab256149) is combined with 10 μM of fibrils (Recombinant Human Alpha-synuclein (mutated A53T) protein aggregate Type 1 (Active) ab256150) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.

  • SDS-PAGE - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (ab256149), expandable thumbnail

    SDS-PAGE - Recombinant human Alpha-synuclein (mutated A53T) protein monomer Type 1 (Active) (ab256149)

    SDS-PAGE - Recombinant Alpha-Synuclein Monomer (mutated A53T) protein (ab256149).

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