Recombinant Human Alpha-synuclein (mutated E46K) protein is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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- Cell 167:1469-1480.e122016Gut Microbiota Regulate Motor Deficits and Neuroinflammation in a Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy R Sampson,Justine W Debelius,Taren Thron,Stefan Janssen,Gauri G Shastri,Zehra Esra Ilhan,Collin Challis,Catherine E Schretter,Sandra Rocha,Viviana Gradinaru,Marie-Francoise Chesselet,Ali Keshavarzian,Kathleen M Shannon,Rosa Krajmalnik-Brown,Pernilla Wittung-Stafshede,Rob Knight,Sarkis K MazmanianPubMed 27912057
- The Journal of biological chemistry 291:24364-243762016Psoralen and Ultraviolet A Light Treatment Directly Affects Phosphatidylinositol 3-Kinase Signal Transduction by Altering Plasma Membrane Packing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBritt Van Aelst et. al.PubMed 27687726
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M D V F M K G L S K / A K E G V V A A A E / K T K Q G V A E A A / G K T K E G V L Y V / G S K T K [ K ] G V V H / G V A T V A E K T K / E Q V T N V G G A V / V T G V T A V A Q K / T V E G A G S I A A / A T G F V K K D Q L / G K N E E G A P Q E / G I L E D M P V D P / D N E A Y E M P S E / E G Y Q D Y E P E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Alternative names
NACP, PARK1, PARK1, NACP, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
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Recombinant Human Alpha-synuclein (mutated E46K) protein is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P37840-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
M D V F M K G L S K / A K E G V V A A A E / K T K Q G V A E A A / G K T K E G V L Y V /
G S K T K [ K ] G V V H / G V A T V A E K T K / E Q V T N V G G A V / V T G V T A V A Q K / T V E G A G S I A A /
A T G F V K K D Q L / G K N E E G A P Q E / G I L E D M P V D P / D N E A Y E M P S E / E G Y Q D Y E P E A- Accession
- Protein length
Full Length
- Predicted molecular weight
14.4 kDa
- Amino acids
1 to 140
- Nature
Recombinant
Specifications
- Form
Liquid
- Additional notes
Alpha Synuclein E46K was overexpressed in *E. coli* and purified to apparent homogeneity by using conventional column chromatography techniques.
General info
- Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
- Sequence similarities
Belongs to the synuclein family.
- Post-translational modifications
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Recent studies have shown that this mutant(E46K) of alpha-synuclein causes Parkinson and Lewy Body dementia(DLB).
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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SDS-PAGE - Recombinant Human Alpha-synuclein (mutated E46K) protein (ab51188)
ab51188 in 15%SDS-PAGE
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