Skip to main content

Recombinant Human Alpha-synuclein protein aggregate (Active) is a Human Full Length SNCA protein, in the 1 to 140 aa range with >95% purity, <= 20 EU/mL endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab218819 recombinant protein is 14.5 kDa.

- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use recombinant Alpha-synuclein (SNCA) protein
- Optimal protein bioactivity, stability and reproducibility

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (AB218819), expandable thumbnail
  • Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Active) (AB218819), expandable thumbnail
  • Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (AB218819), expandable thumbnail
  • SDS-PAGE - Recombinant Human Alpha-synuclein protein aggregate (Active) (AB218819), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (AB218819), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
<= 20 EU/mL
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS, WB
Biologically active
Yes

Amino acid sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Recombinant Human Alpha-synuclein protein aggregate (Active) is a Human Full Length SNCA protein, in the 1 to 140 aa range with >95% purity, <= 20 EU/mL endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab218819 recombinant protein is 14.5 kDa.

- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use recombinant Alpha-synuclein (SNCA) protein
- Optimal protein bioactivity, stability and reproducibility

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
<= 20 EU/mL
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS, WB
Biological activity
Endogenous alpha-synuclein phosphorylation.

100 μM alpha synuclein protein monomer (ab218818) seeded with 10 μM alpha synuclein protein aggregate (ab218819) in 25 μM Thioflavin T (ab120751) (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.

Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.

Endotoxin Level: 10-20 EU/mL

Accession
P37840-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

Constituents: 95% PBS

Sequence info

Amino acid sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
Accession
P37840
Protein length
Full Length
Predicted molecular weight
14.46 kDa
Amino acids
1 to 140
Nature
Recombinant

Specifications

Form
Liquid
Additional notes

ab218819 was purified by ion-exchange.

General info

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity.

This supplementary information is collated from multiple sources and compiled automatically.

Sequence similarities

Belongs to the synuclein family.

Post-translational modifications

Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Ensure the validity of your result using our Recombinant Alpha-synuclein (SNCA) protein Native Human IgG FC fragment protein ab90285 as a control.
ab218819 can be used as a positive control in SDS-PAGE and western blot assays.

Check out our Thioflavin T assay protocol here.

For best results, sonicate ab218819 immediately prior to use. Sonication of PFFs is required in order to obtain fibrils with an average length of ~50nm, the optimal size for pathology.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    Immunohistochemical analysis of primary rat hippocampal neurons showing lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate (ab218819) at 4 μg/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate (Recombinant Human Alpha-synuclein protein aggregate (Type 2) ab218817) at 4 μg/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary antibody: Mouse anti-pSer129 Antibody at 1/1000 24 hours at 4°C. Secondary antibody: FITC Goat Anti-Mouse (green) at 1/700 for 1 hour at RT. Counterstain: Hoechst (blue) nuclear stain at 1/4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.

  • Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    ab218819 seeds the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers.

    Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity.

    Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 μM of ab218819 is combined with 100 μM of alpha synuclein monomer, as compared to ab218819 alone and alpha synuclein monomer alone.

    Thioflavin T ex = 450 nm, em = 485 nm.

  • Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    TEM of active human alpha synuclein preformed fibrils (ab218819). Fibrils were sonicated and treated with uranyl acetate.

  • SDS-PAGE - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    SDS-PAGE - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    SDS-PAGE analysis of ab218819.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    Immunohistochemistry analysis of rat brain injected with active human alpha synuclein PFFs (ab218819). Species: Female Sprague-Dawley Rat. Rat was injected with 2μL active human alpha synuclein PFFs (ab218819) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Alpha synuclein pathology is seen in the striatum close to an injection site.

  • Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    TEM of ab218819.

  • Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    TEM of active human alpha synuclein preformed fibrils (ab218819) (top) and control (inactive) human alpha synuclein preformed fibrils (Recombinant Human Alpha-synuclein protein aggregate (Type 2) ab218817) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the inactive fibrils.

  • Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    ThT emission curves show increased fluorescence (correlated to alpha-synucleinprotein aggregation) over time when 10 μM of active alpha-synuclein aggregate (ab218819) is combined with 100 μM of active alpha-synuclein monomer (Recombinant Human Alpha-synuclein protein monomer (Active) ab218818) (light blue), as compared to when 100 μM of active alpha-synuclein monomer is combined with 10 μM of control alpha-synuclein aggregate (purple line), or 100 μM of control alpha-synuclein monomer (Recombinant Human Alpha-synuclein protein monomer (Type 2) ab218816) is combined with 10 μM of control alpha-synuclein aggregate (Recombinant Human Alpha-synuclein protein aggregate (Type 2) ab218817) (dark blue). ThT ex = 450 nm, em = 485 nm. View protocol.

  • Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819), expandable thumbnail

    Electron Microscopy - Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    TEM of active human alpha synuclein preformed fibrils (ab218819) (top) and control (inactive) human alpha synuclein preformed fibrils (Recombinant Human Alpha-synuclein protein aggregate (Type 2) ab218817) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the inactive fibrils.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com