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Recombinant Human Alpha-synuclein protein aggregate (Active) is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity, <= 20 EU/mL endotoxin level and suitable for SDS-PAGE, FuncS, WB.

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Purity

>95% SDS-PAGE

Endotoxin level

<= 20 EU/mL

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS, WB

Biologically active

Yes

Sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Human Alpha-synuclein protein aggregate (Active) is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity, <= 20 EU/mL endotoxin level and suitable for SDS-PAGE, FuncS, WB.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<= 20 EU/mL

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS, WB

Biological activity

Endogenous alpha-synuclein phosphorylation.

100 μM alpha synuclein protein monomer (ab218818) seeded with 10 μM alpha synuclein protein aggregate (ab218819) in 25 μM Thioflavin T (ab120751) (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.

Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.

Endotoxin Level: 10-20 EU/mL

Predicted molecular weight

14.46 kDa

Amino acids

1 to 140

Sequence

M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A

Accession
P37840-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

Constituents: 95% PBS

Specifications

Form

Liquid

Additional notes

ab218819 was purified by ion-exchange.

General info

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Sequence similarities

Belongs to the synuclein family.

Post-translational modifications

Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

For best results, sonicate immediately prior to use. Sonication of PFFs is required in order to obtain fibrils with an average length of ~50nm, the optimal size for pathology.

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Product protocols

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