Recombinant human Alpha-Synuclein protein filament is a Human Full Length protein, in the 1 to 140 aa range with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, ELISA and Dot blot. The predicted molecular weight of ab254309 protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our human Alpha-synuclein (SNCA) protein as a control
- Optimal protein bioactivity and stability
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, Dot, sELISA
- Biologically active
- Yes
Amino acid sequence
M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Recombinant human Alpha-Synuclein protein filament is a Human Full Length protein, in the 1 to 140 aa range with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, ELISA and Dot blot. The predicted molecular weight of ab254309 protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our human Alpha-synuclein (SNCA) protein as a control
- Optimal protein bioactivity and stability
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, Dot, sELISA
- Biologically active
- Yes
- Biological activity
- Functional in a thioflavin binding assay.
>x100 signal when compared to monomer control.
- Accession
- P37840-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
Sequence info
Amino acid sequence
- M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
- Accession
- P37840
- Protein length
- Full Length
- Amino acids
- 1 to 140
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity >95% by LC/MS
General info
- Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the synuclein family.
- Post-translational modifications
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant human Alpha-synuclein protein filament ab254309 as a control in SDS-PAGE and Dot blot assays.
Analyze your alpha-synuclein (SNCA) ELISA data using the ab254309 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE here
Check out our western blot protocol for more information here
Protocol FAQ
Q: Do you have a recommended protocol for the Thioflavin T Fluorescence assay?
A: Please check our recommended Thioflavin T Fluorescence assay here
Function
Alpha-Synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
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4 product images
Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament (ab254309)
Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament standard curve
Background subtracted standard curve using Human Alpha-synuclein Antibody Pair - BSA and Azide free (Human Alpha-synuclein Antibody Pair - BSA and Azide free ab270346) and Recombinant human Alpha-Synuclein protein filament (ab254309) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Functional Studies - Recombinant human Alpha-Synuclein protein filament (ab254309)
Thioflavin binding by ab254309.
Functional in a thioflavin binding assay.
>x100 signal when compared to monomer control.
SDS-PAGE - Recombinant human Alpha-Synuclein protein filament (ab254309)
SDS-PAGE analysis of ab254309.
Dot Blot - Recombinant human Alpha-Synuclein protein filament (ab254309)
Confirmation specific Antibody binding.
Positive signal when assayed by Dotblot, binding Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab209538.
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