Recombinant Human Alpha-synuclein protein monomer (Active) is a Human Full Length SNCA protein, in the 1 to 140 aa range with >95% purity, < 5 EU/mL endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab218818 recombinant protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our recombinant Alpha-synuclein (SNCA) protein
- Optimal protein bioactivity, stability and reproducibility
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- Life science alliance 7:2024Nr1h4 and Thrb ameliorate ER stress and provide protection in the MPTP mouse model of Parkinson's.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNancy Ahuja,Shalini Gupta,Rashmi Arora,Ella Bhagyaraj,Drishti Tiwari,Sumit Kumar,Pawan GuptaPubMed 38609183
- NPJ Parkinson's disease 8:1152022Long-term inhibition of mutant LRRK2 hyper-kinase activity reduced mouse brain α-synuclein oligomers without adverse effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilip Wing-Lok Ho,Eunice Eun-Seo Chang,Chi-Ting Leung,Huifang Liu,Yasine Malki,Shirley Yin-Yu Pang,Zoe Yuen-Kiu Choi,Yingmin Liang,Weng Seng Lai,Yuefei Ruan,Kenneth Mei-Yee Leung,Susan Yung,Judith Choi-Wo Mak,Michelle Hiu-Wai Kung,David B Ramsden,Shu-Leong HoPubMed 36088364
- Biomolecules 12:2022Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBuket Uçar,Nadia Stefanova,Christian HumpelPubMed 35204664
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 5 EU/mL
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
Amino acid sequence
M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Recombinant Human Alpha-synuclein protein monomer (Active) is a Human Full Length SNCA protein, in the 1 to 140 aa range with >95% purity, < 5 EU/mL endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab218818 recombinant protein is 14.5 kDa.
- Suitable for Thioflavin T Fluorescence assay
- Save time and ensure accurate results - use our recombinant Alpha-synuclein (SNCA) protein
- Optimal protein bioactivity, stability and reproducibility
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 5 EU/mL
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
- Biological activity
- 100 μM alpha synuclein protein monomer (ab218818), seeded with 10 μM alpha synuclein protein aggregate in 25 μM Thioflavin T (ab120751) (PBS pH 7.4, 100 μl reaction volume), generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.
Fluorescence was measured by excitation at 450 nm and emission at 485 nm, on a Molecular Devices Gemini XPS microplate reader.
Endotoxin Level: < 5 EU/mL
- Accession
- P37840-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M D V F M K G L S K A K E G V V A A A E K T K Q G V A E A A G K T K E G V L Y V G S K T K E G V V H G V A T V A E K T K E Q V T N V G G A V V T G V T A V A Q K T V E G A G S I A A A T G F V K K D Q L G K N E E G A P Q E G I L E D M P V D P D N E A Y E M P S E E G Y Q D Y E P E A
- Accession
- P37840
- Protein length
- Full Length
- Predicted molecular weight
- 14.46 kDa
- Amino acids
- 1 to 140
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab218818 is purified by ion exchange.
General info
- Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the synuclein family.
- Post-translational modifications
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our Recombinant Human Alpha-synuclein (SCNA) protein monomer (Active) ab218818 as a control in western blot and SDS-PAGE.
Check out of western blot protocol for more information here
Protocol FAQ
Q: Do you have a recommended protocol for the Thioflavin T Fluorescence assay?
A: Please check our recommended Thioflavin T Fluorescence assay here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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2 product images
SDS-PAGE - Recombinant Human Alpha-synuclein protein monomer (Active) (ab218818)
SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer (SPR-321). Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 µg) (SPR-321).
Functional Studies - Recombinant Human Alpha-synuclein protein monomer (Active) (ab218818)
ThT emission curves show increased fluorescence (correlated to alpha-synuclein protein aggregation) over time when 10 μM of active alpha-synuclein aggregate (Recombinant Human Alpha-synuclein protein aggregate (Active) ab218819) is combined with 100 μM of active alpha-synuclein monomer (ab218818) (light blue), as compared to when 100 μM of control alpha-synuclein monomer (Recombinant Human Alpha-synuclein protein monomer (Type 2) ab218816) is combined with 10 μM of active alpha-synuclein aggregate (Recombinant Human Alpha-synuclein protein aggregate (Active) ab218819) (dark blue).
ThT ex = 450 nm, em = 485 nm. View protocol.
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