Recombinant Human AMF protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Publications
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- Molecular neurobiology 56:1558-15772018Oxidation Resistance 1 Modulates Glycolytic Pathways in the Cerebellum via an Interaction with Glucose-6-Phosphate Isomerase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMattéa J Finelli et. al.PubMed 29905912
- Molecular medicine reports 13:67-742015Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKang Tian,Weiliang Zhong,Yingqiu Zhang,Baosheng Yin,Weiguo Zhang,Han LiuPubMed 26573126
- Arthritis research & therapy 17:1002015Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zong et. al.PubMed 25889507
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A A L T R D P Q F Q K L Q Q W Y R E H R S E L N L R R L F D A N K D R F N H F S L T L N T N H G H I L V D Y S K N L V T E D V M R M L V D L A K S R G V E A A R E R M F N G E K I N Y T E G R A V L H V A L R N R S N T P I L V D G K D V M P E V N K V L D K M K S F C Q R V R S G D W K G Y T G K T I T D V I N I G I G G S D L G P L M V T E A L K P Y S S G G P R V W Y V S N I D G T H I A K T L A Q L N P E S S L F I I A S K T F T T Q E T I T N A E T A K E W F L Q A A K D P S A V A K H F V A L S T N T T K V K E F G I D P Q N M F E F W D W V G G R Y S L W S A I G L S I A L H V G F D N F E Q L L S G A H W M D Q H F R T T P L E K N A P V L L A L L G I W Y I N C F G C E T H A M L P Y D Q Y L H R F A A Y F Q Q G D M E S N G K Y I T K S G T R V D H Q T G P I V W G E P G T N G Q H A F Y Q L I H Q G T K M I P C D F L I P V Q T Q H P I R K G L H H K I L L A N F L A Q T E A L M R G K S T E E A R K E L Q A A G K S P E D L E R L L P H K V F E G N R P T N S I V F T K L T P F M L G A L V A M Y E H K I F V Q G I I W D I N S F D Q W G V E L G K Q L A K K I E P E L D G S A Q V T S H D A S T N G L I N F I K Q Q R E A R V Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab87625 can be used as a WB positive control in conjunction with ab86950. |
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Target data
Function
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:11004567, PubMed:3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:11004567, PubMed:3352745).
Alternative names
Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36
Recommended products
Recombinant Human AMF protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P06744-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A A L T R D P Q F Q K L Q Q W Y R E H R S E L N L R R L F D A N K D R F N H F S L T L N T N H G H I L V D Y S K N L V T E D V M R M L V D L A K S R G V E A A R E R M F N G E K I N Y T E G R A V L H V A L R N R S N T P I L V D G K D V M P E V N K V L D K M K S F C Q R V R S G D W K G Y T G K T I T D V I N I G I G G S D L G P L M V T E A L K P Y S S G G P R V W Y V S N I D G T H I A K T L A Q L N P E S S L F I I A S K T F T T Q E T I T N A E T A K E W F L Q A A K D P S A V A K H F V A L S T N T T K V K E F G I D P Q N M F E F W D W V G G R Y S L W S A I G L S I A L H V G F D N F E Q L L S G A H W M D Q H F R T T P L E K N A P V L L A L L G I W Y I N C F G C E T H A M L P Y D Q Y L H R F A A Y F Q Q G D M E S N G K Y I T K S G T R V D H Q T G P I V W G E P G T N G Q H A F Y Q L I H Q G T K M I P C D F L I P V Q T Q H P I R K G L H H K I L L A N F L A Q T E A L M R G K S T E E A R K E L Q A A G K S P E D L E R L L P H K V F E G N R P T N S I V F T K L T P F M L G A L V A M Y E H K I F V Q G I I W D I N S F D Q W G V E L G K Q L A K K I E P E L D G S A Q V T S H D A S T N G L I N F I K Q Q R E A R V Q
- Accession
- P06744
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab87625 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).
General info
- Function
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:11004567, PubMed:3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:11004567, PubMed:3352745).
- Sequence similarities
Belongs to the GPI family.
- Post-translational modifications
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Glucose 6 phosphate isomerase
Product promise
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2 product images
SDS-PAGE - Recombinant Human AMF protein (ab87625)
15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).
Western blot - Recombinant Human AMF protein (ab87625)
All lanes: Anti-AMF antibody (ab86950) at 1 µg/mL
Lane 1: Western blot - Recombinant Human AMF protein (ab87625) at 0.1 µg
Lane 2: Western blot - Recombinant Human AMF protein (ab87625) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30s
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