JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB87625

Recombinant Human AMF protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(3 Publications)

Recombinant Human AMF protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36

2 Images
Western blot - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)
  • WB

Unknown

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)

All lanes:

Anti-AMF antibody (<a href='/en-us/products/unavailable/amf-antibody-ab86950'>ab86950</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (ab87625) at 0.1 µg

Lane 2:

Western blot - Recombinant Human AMF protein (His tag N-Terminus) (ab87625) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 30s

SDS-PAGE - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AMF protein (His tag N-Terminus) (AB87625)

15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P06744

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab87625 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/amf-antibody-ab86950'>ab86950</a>.</p>" } } }
style
left-column

Product details

This product was previously labelled as Glucose 6 phosphate isomerase
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06744","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab87625 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method).

style
left-column

General info

Function

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).

Sequence similarities

Belongs to the GPI family.

Post-translational modifications

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.. ISGylated.

style
left-column

Product protocols

style
left-column

Target data

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).
See full target information GPI
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 56:1558-1577 PubMed29905912

2018

Oxidation Resistance 1 Modulates Glycolytic Pathways in the Cerebellum via an Interaction with Glucose-6-Phosphate Isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Mattéa J Finelli,Teresa Paramo,Elisabete Pires,Brent J Ryan,Richard Wade-Martins,Philip C Biggin,James McCullagh,Peter L Oliver

Molecular medicine reports 13:67-74 PubMed26573126

2015

Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Tian,Weiliang Zhong,Yingqiu Zhang,Baosheng Yin,Weiguo Zhang,Han Liu

Arthritis research & therapy 17:100 PubMed25889507

2015

Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zong,Tianbao Lu,Shasha Fan,Hui Zhang,Ruhan Gong,Lishan Sun,Zhiyan Fu,Lieying Fan
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB171575

Human Glucose-6-Phosphate Isomerase ELISA Kit

SimpleStep

Sandwich ELISA - Human Glucose-6-Phosphate Isomerase ELISA Kit (AB171575)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB313590

Anti-AMF antibody [EPR28372-33] - BSA and Azide free

BOND RX™ Validated|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR28372-33] - BSA and Azide free (AB313590)

  • 0
  • 0
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com