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Recombinant human AMF protein (His tag) is a Human Full Length protein, in the 2 to 558 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.

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Images

HPLC - Recombinant human AMF protein (His tag) (AB318251), expandable thumbnail
  • Mass Spectrometry - Recombinant human AMF protein (His tag) (AB318251), expandable thumbnail

Key facts

Purity
>=95% HPLC
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Tags
His tag N-Terminus
Applications
MS, HPLC, SDS-PAGE
Biologically active
No
Mass spectrometry
LC-MS/MS

Amino acid sequence

A A L T R D P Q F Q K L Q Q W Y R E H R S E L N L R R L F D A N K D R F N H F S L T L N T N H G H I L V D Y S K N L V T E D V M R M L V D L A K S R G V E A A R E R M F N G E K I N Y T E G R A V L H V A L R N R S N T P I L V D G K D V M P E V N K V L D K M K S F C Q R V R S G D W K G Y T G K T I T D V I N I G I G G S D L G P L M V T E A L K P Y S S G G P R V W Y V S N I D G T H I A K T L A Q L N P E S S L F I I A S K T F T T Q E T I T N A E T A K E W F L Q A A K D P S A V A K H F V A L S T N T T K V K E F G I D P Q N M F E F W D W V G G R Y S L W S A I G L S I A L H V G F D N F E Q L L S G A H W M D Q H F R T T P L E K N A P V L L A L L G I W Y I N C F G C E T H A M L P Y D Q Y L H R F A A Y F Q Q G D M E S N G K Y I T K S G T R V D H Q T G P I V W G E P G T N G Q H A F Y Q L I H Q G T K M I P C D F L I P V Q T Q H P I R K G L H H K I L L A N F L A Q T E A L M R G K S T E E A R K E L Q A A G K S P E D L E R L L P H K V F E G N R P T N S I V F T K L T P F M L G A L V A M Y E H K I F V Q G I I W D I N S F D Q W G V E L G K Q L A K K I E P E L D G S A Q V T S H D A S T N G L I N F I K Q Q R E A R V Q

Reactivity data

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:11004567, PubMed:3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:11004567, PubMed:3352745).

Alternative names

Recommended products

Recombinant human AMF protein (His tag) is a Human Full Length protein, in the 2 to 558 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.

Key facts

Purity
>=95% HPLC
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Applications
MS, HPLC, SDS-PAGE
Accession
P06744-1
Animal free
Yes
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.3752% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

A A L T R D P Q F Q K L Q Q W Y R E H R S E L N L R R L F D A N K D R F N H F S L T L N T N H G H I L V D Y S K N L V T E D V M R M L V D L A K S R G V E A A R E R M F N G E K I N Y T E G R A V L H V A L R N R S N T P I L V D G K D V M P E V N K V L D K M K S F C Q R V R S G D W K G Y T G K T I T D V I N I G I G G S D L G P L M V T E A L K P Y S S G G P R V W Y V S N I D G T H I A K T L A Q L N P E S S L F I I A S K T F T T Q E T I T N A E T A K E W F L Q A A K D P S A V A K H F V A L S T N T T K V K E F G I D P Q N M F E F W D W V G G R Y S L W S A I G L S I A L H V G F D N F E Q L L S G A H W M D Q H F R T T P L E K N A P V L L A L L G I W Y I N C F G C E T H A M L P Y D Q Y L H R F A A Y F Q Q G D M E S N G K Y I T K S G T R V D H Q T G P I V W G E P G T N G Q H A F Y Q L I H Q G T K M I P C D F L I P V Q T Q H P I R K G L H H K I L L A N F L A Q T E A L M R G K S T E E A R K E L Q A A G K S P E D L E R L L P H K V F E G N R P T N S I V F T K L T P F M L G A L V A M Y E H K I F V Q G I I W D I N S F D Q W G V E L G K Q L A K K I E P E L D G S A Q V T S H D A S T N G L I N F I K Q Q R E A R V Q
Accession
P06744
Protein length
Full Length
Predicted molecular weight
64.92 kDa
Amino acids
2 to 558
Nature
Recombinant
Tags
His tag N-Terminus

Specifications

Form
Liquid

General info

Function

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:11004567, PubMed:3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:11004567, PubMed:3352745).

Sequence similarities

Belongs to the GPI family.

Post-translational modifications

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • HPLC - Recombinant human AMF protein (His tag) (ab318251), expandable thumbnail

    HPLC - Recombinant human AMF protein (His tag) (ab318251)

    HPLC analysis of ab318251

  • Mass Spectrometry - Recombinant human AMF protein (His tag) (ab318251), expandable thumbnail

    Mass Spectrometry - Recombinant human AMF protein (His tag) (ab318251)

    Mass determination by ESI-TOF.
    Predicted MW is 64920.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 64918.99 Da.

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Product protocols

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