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AB318251

Recombinant human AMF protein (His tag)

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Recombinant human AMF protein (His tag) is a Human Full Length protein, in the 2 to 558 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, HPLC, SDS-PAGE.

View Alternative Names

Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, D-hexose-6-phosphate anomerase, Glucose-6-phosphate C2-epimerase, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36

2 Images
Mass Spectrometry - Recombinant human AMF protein (His tag) (AB318251)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human AMF protein (His tag) (AB318251)

Mass determination by ESI-TOF. Predicted MW is 64920.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 64918.99 Da.

HPLC - Recombinant human AMF protein (His tag) (AB318251)
  • HPLC

Supplier Data

HPLC - Recombinant human AMF protein (His tag) (AB318251)

HPLC analysis of ab318251

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

HPLC, SDS-PAGE, Mass Spec

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

P06744

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.3752% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Assay Kits

AB171575

Human Glucose-6-Phosphate Isomerase ELISA Kit

SimpleStep

Sandwich ELISA - Human Glucose-6-Phosphate Isomerase ELISA Kit (AB171575)

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  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB313590

Anti-AMF antibody [EPR28372-33] - BSA and Azide free

BOND RX™ Validated|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR28372-33] - BSA and Azide free (AB313590)

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Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ","proteinLength":"Full Length","predictedMolecularWeight":"64.92 kDa","actualMolecularWeight":"64.92 kDa","aminoAcidEnd":558,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P06744","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Specifications

Form

Liquid

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General info

Function

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).

Sequence similarities

Belongs to the GPI family.

Post-translational modifications

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.. ISGylated.

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Product protocols

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Target data

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).
See full target information GPI
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Product promise

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