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AB229600

Recombinant human Amphiregulin protein (Active)

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Recombinant human Amphiregulin protein (Active) is a Human Full Length protein, in the 101 to 187 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

2 Images
Functional Studies - Recombinant human Amphiregulin protein (Active) (AB229600)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Amphiregulin protein (Active) (AB229600)

ab229600 induced proliferation of 3T3 cells.

SDS-PAGE - Recombinant human Amphiregulin protein (Active) (AB229600)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Amphiregulin protein (Active) (AB229600)

1 μg ab229600 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.

Lane 1 : Non-reducing conditions.

Lane 2 : Reducing conditions.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

3T3 Cell Proliferation.

ED50 ≤ 20 ng/ml (≥ 5.0 x 104 units/mg).

Accession

P15514

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.16% Sodium phosphate

storage-buffer

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Complementary Products

Primary Antibodies

AB180722

Anti-Amphiregulin antibody

Western blot - Anti-Amphiregulin antibody (AB180722)

  • 4
  • 3
Primary Antibodies

AB133582

Anti-CD73 antibody [EPR6114]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR6114] (AB133582)

  • 3
  • 2
Assay Kits

AB222504

Human Amphiregulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Amphiregulin ELISA Kit (AB222504)

  • 0
  • 0
Primary Antibodies

AB134152

Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMK","proteinLength":"Full Length","predictedMolecularWeight":"10.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":101,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15514","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
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Specifications

Form

Lyophilized

Additional notes

Purity determined by reducing and non-reducing SDS-PAGE.

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General info

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Sequence similarities

Belongs to the amphiregulin family.

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Product protocols

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Target data

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
See full target information AREG
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Product promise

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