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AB79803

Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein

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(4 Publications)

Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

AMPK, PRKAB1, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb, AMPK1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, ACACA kinase, HMGCR kinase, 5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1

2 Images
Functional Studies - Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (AB79803)
  • FuncS

Unknown

Functional Studies - Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (AB79803)

Image showing specific activity of ab79803.

SDS-PAGE - Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (AB79803)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human AMPK alpha1 + AMPK beta1 + AMPK gamma1 protein (AB79803)

10% SDS-PAGE, Coomassie staining.
Lane 1 : Protein marker.
Lane 2 : ab79803

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: 2755 nmol/min/mg.

Accession

Q9Y478

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Proteins & Peptides

AB184883

Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active)

Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (AB184883)

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  • 0
Primary Antibodies

AB32047

Anti-AMPK alpha 1 antibody [Y365]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-AMPK alpha 1 antibody [Y365] (AB32047)

  • 5
  • 6
Proteins & Peptides

AB202187

Recombinant Human LKB1 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human LKB1 protein (denatured) (His tag N-Terminus) (AB202187)

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  • 0
Proteins & Peptides

AB62572

Recombinant Human MNK1 protein (GST tag N-Terminus)

Western blot - Recombinant Human MNK1 protein (GST tag N-Terminus) (AB62572)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"HHHHHHLVPRGSPEFMRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQFEPSGRPVLHHHHHHMGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPIFEPSGRPVLHHHHHHMETVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVVLRALSCPLGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKPFEPSGRPVLHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y478","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Sequence similarities

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Post-translational modifications

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

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Product protocols

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Target data

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
See full target information PRKAB1

Additional targets

PRKAG1,PRKAA1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 79:934-949.e14 PubMed32822587

2020

Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls Adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Huang,Cristel V Camacho,Rohit Setlem,Keun Woo Ryu,Balaji Parameswaran,Rana K Gupta,W Lee Kraus

Nature communications 9:4728 PubMed30413706

2018

The critical role of AMPK in driving Akt activation under stress, tumorigenesis and drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Han,Chien-Feng Li,Zhen Cai,Xian Zhang,Guoxiang Jin,Wei-Na Zhang,Chuan Xu,Chi-Yun Wang,John Morrow,Shuxing Zhang,Dazhi Xu,Guihua Wang,Hui-Kuan Lin

Kidney international 93:81-94 PubMed28739141

2017

AMP-activated protein kinase/myocardin-related transcription factor-A signaling regulates fibroblast activation and renal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuguo Wang,Li Jia,Zhaoyong Hu,Mark L Entman,William E Mitch,Yanlin Wang

Nature cell biology 18:431-42 PubMed26999735

2016

LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Liu,Zhen-Dong Xiao,Leng Han,Jiexin Zhang,Szu-Wei Lee,Wenqi Wang,Hyemin Lee,Li Zhuang,Junjie Chen,Hui-Kuan Lin,Jing Wang,Han Liang,Boyi Gan
View all publications
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Product promise

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