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AB113582

Recombinant Human AMPS protein

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Recombinant Human AMPS protein is a Human Full Length protein, in the 1 to 484 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

AMPS, ADSL, Adenylosuccinate lyase, ASL, Adenylosuccinase, ASase

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SDS-PAGE - Recombinant Human AMPS protein (AB113582)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AMPS protein (AB113582)

15% SDS-PAGE showing ab113582 at approximately 59kDa (3µg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P30566

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Adenylosuccinate Lyase.
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMAAGGDHGSPDSYRSPLASRYASPEMCFVFSDRYKFRTWRQLWLWLAEAEQTLGLPITDEQIQEMKSNLENIDFKMAAEEEKRLRHDVMAHVHTFGHCCPKAAGIIHLGATSCYVGDNTDLIILRNALDLLLPKLARVISRLADFAKERASLPTLGFTHFQPAQLTTVGKRCCLWIQDLCMDLQNLKRVRDDLRFRGVKGTTGTQASFLQLFEGDDHKVEQLDKMVTEKAGFKRAFIITGQTYTRKVDIEVLSVLASLGASVHKICTDIRLLANLKEMEEPFEKQQIGSSAMPYKRNPMRSERCCSLARHLMTLVMDPLQTASVQWFERTLDDSANRRICLAEAFLTADTILNTLQNISEGLVVYPKVIERRIRQELPFMATENIIMAMVKAGGSRQDCHEKIRVLSQQAASVVKQEGGDNDLIERIQVDAYFSPIHSQLDHLLDPSSFTGRASQQVQRFLEEEVYPLLKPYESVMKVKAELCL","proteinLength":"Full Length","predictedMolecularWeight":"59 kDa","actualMolecularWeight":null,"aminoAcidEnd":484,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30566","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab113582 is purified using conventional chromatography techniques: anion exchange chromatography followed by gel filtration chromatography with 20mM Tris pH 7.5, 2mM EDTA. No detergents such as urea, Triton or Tween etc. were used to purify the proteins.

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General info

Function

Catalyzes two non-sequential steps in de novo AMP synthesis : converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.

Sequence similarities

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

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Product protocols

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Target data

Catalyzes two non-sequential steps in de novo AMP synthesis : converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.
See full target information ADSL
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Complementary Products

Primary Antibodies

AB249171

Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (AB249171)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB129191

Anti-PGAM1 antibody [EPR8042]

RabMAb|Recombinant

Western blot - Anti-PGAM1 antibody [EPR8042] (AB129191)

  • 5
  • 1
Primary Antibodies

AB24733

Anti-GC1q R antibody [60.11]

BOND RX™ Validated|Trial Size

Western blot - Anti-GC1q R antibody [60.11] (AB24733)

  • 5
  • 3

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