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AB268347

Recombinant human AMSH protein (Active)

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Recombinant human AMSH protein (Active) is a Human Full Length protein, in the 1 to 424 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

AMSH, STAMBP, STAM-binding protein, Associated molecule with the SH3 domain of STAM, Endosome-associated ubiquitin isopeptidase

2 Images
Functional Studies - Recombinant human AMSH protein (Active) (AB268347)
  • FuncS

Supplier Data

Functional Studies - Recombinant human AMSH protein (Active) (AB268347)

The specific activity of ab268347 was ≥12 nmol/min/min in a deubiquitinase assay using recombinant human ubiquitin-based proluciferin as substrate.

SDS-PAGE - Recombinant human AMSH protein (Active) (AB268347)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human AMSH protein (Active) (AB268347)

SDS-PAGE analysis of ab268347.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268347 was ≥12 nmol/min/min in a deubiquitinase assay using recombinant human ubiquitin-based proluciferin as substrate.

Accession

O95630

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAFPKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVAQQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDLEKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":424,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O95630","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed : 15314065, PubMed : 23542699, PubMed : 34425109). Does not cleave 'Lys-48'-linked polyubiquitin chains (PubMed : 15314065). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF (PubMed : 10383417). Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 (PubMed : 11483516). Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes (PubMed : 15314065, PubMed : 17261583). Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (PubMed : 15314065, PubMed : 17261583). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways (PubMed : 23542699).

Sequence similarities

Belongs to the peptidase M67C family.

Post-translational modifications

Phosphorylated after BMP type I receptor activation.. Ubiquitinated by SMURF2 in the presence of RNF11.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed : 15314065, PubMed : 23542699, PubMed : 34425109). Does not cleave 'Lys-48'-linked polyubiquitin chains (PubMed : 15314065). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF (PubMed : 10383417). Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 (PubMed : 11483516). Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes (PubMed : 15314065, PubMed : 17261583). Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (PubMed : 15314065, PubMed : 17261583). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways (PubMed : 23542699).
See full target information STAMBP
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