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AB127267

Recombinant Human ANAPC2 protein (His-DHFR)

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Recombinant Human ANAPC2 protein (His-DHFR) is a Human Fragment protein, in the 604 to 820 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

APC2, KIAA1406, ANAPC2, Anaphase-promoting complex subunit 2, Cyclosome subunit 2

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UJX6

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"25 kDa","actualMolecularWeight":null,"aminoAcidEnd":820,"aminoAcidStart":604,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UJX6","tags":[{"tag":"His-DHFR","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 11739784, PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 11739784, PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity).

Sequence similarities

Belongs to the cullin family.

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Product protocols

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Target data

Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 11739784, PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 11739784, PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity).
See full target information ANAPC2
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Product promise

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