Recombinant Human Androgen receptor (Isoform 3) Protein Standard (His tag)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human Androgen receptor (Isoform 3) Protein Standard (His tag) AB317678 is a Human Fragment protein expressed in Escherichia coli suitable for SDS-PAGE, sELISA.
- Localization: Intracellular
- Proximate region: C-terminus
- His tag C-Terminus
- >80% SDS-PAGE
View Alternative Names
DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
- SDS-PAGE
Lab
SDS-PAGE - Recombinant Human Androgen receptor (Isoform 3) Protein Standard (His tag) (AB317678)
SDS-PAGE analysis of ab317678 under reducing conditions for 2ug protein.
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform.
Sequence info
Properties and storage information
Form
Purification technique
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The AR-V7 contributes to the regulation of genes typically involved in cell growth and survival. AR-V7 operates as part of the androgen receptor complex which translocates into the nucleus to influence gene transcription. Due to its persistent activity AR-V7 can drive the expression of androgen-responsive genes which contributes to the proliferation of cells even in low androgen environments. This activity shows why AR-V7 presence creates therapeutic challenges because it promotes unnecessary cell growth.
Pathways
AR-V7 interacts within the androgen signaling pathway which regulates androgen-responsive gene expression. A significant relationship exists between AR-V7 and proteins like coactivators which enhance its transcriptional activity. By bypassing the typical inhibition provided by androgen deprivation therapies (ADTs) AR-V7 sustains pathway activities leading to continued cancer cell survival and proliferation. It is also related to the Akt pathway which further supports cell survival and metabolic processes.
General info
Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed : 19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.. Isoform 3. Lacks the C-terminal ligand-binding domain and therefore constitutively regulates the transcription of a specific set of canonical AR-target genes, including PSA/KLK3 and TMPRSS2, independently of steroid hormones (PubMed : 19244107, PubMed : 25008967). However, some genes are differentially regulated by full-length AR (isoform 1) and isoform 3. Isoform 3-specific target genes may be regulated independently of FOXA1 expression (PubMed : 25008967).. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Post-translational modifications
Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.. Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com