Recombinant Human Androgen Receptor protein (His tag) is a Human Fragment protein, in the 650 to 920 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EMSA, GSA.
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Publications
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- International journal of cancer 145:415-4262019Polycomb group proteins EZH2 and EED directly regulate androgen receptor in advanced prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQipeng Liu et. al.PubMed 30628724
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, EMSA, GSA
- Biologically active
- No
Amino acid sequence
T S P T E E T T Q K L T V S H I E G Y E C Q P I F L N V L E A I E P G V V C A G H D N N Q P D S F A A L L S S L N E L G E R Q L V H V V K W A K A L P G F R N L H V D D Q M A V I Q Y S W M G L M V F A M G W R S F T N V N S R M L Y F A P D L V F N E Y R M H K S R M Y S Q C V R M R H L S Q E F G W L Q I T P Q E F L C M K A L L L F S I I P V D G L K N Q K F F D E L R M N Y I K E L D R I I A C K R K N P T S C S R R F Y Q L T K L L D S V Q P I A R E L H Q F T F D L L I K S H M V S V D F P E M M A E I I S V Q V P K I L S G K V K P I Y F H T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application EMSA | Reactivity Reacts | Dilution info - | Notes - |
Application GSA | Reactivity Reacts | Dilution info - | Notes Can be used for protein-protein and small molecules-protein interaction assays. |
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Target data
Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Alternative names
DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Recommended products
Recombinant Human Androgen Receptor protein (His tag) is a Human Fragment protein, in the 650 to 920 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EMSA, GSA.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, EMSA, GSA
- Biologically active
- No
- Accession
- P10275-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Sequence info
Amino acid sequence
- T S P T E E T T Q K L T V S H I E G Y E C Q P I F L N V L E A I E P G V V C A G H D N N Q P D S F A A L L S S L N E L G E R Q L V H V V K W A K A L P G F R N L H V D D Q M A V I Q Y S W M G L M V F A M G W R S F T N V N S R M L Y F A P D L V F N E Y R M H K S R M Y S Q C V R M R H L S Q E F G W L Q I T P Q E F L C M K A L L L F S I I P V D G L K N Q K F F D E L R M N Y I K E L D R I I A C K R K N P T S C S R R F Y Q L T K L L D S V Q P I A R E L H Q F T F D L L I K S H M V S V D F P E M M A E I I S V Q V P K I L S G K V K P I Y F H T Q
- Accession
- P10275
- Protein length
- Fragment
- Predicted molecular weight
- 33.7 kDa
- Amino acids
- 650 to 920
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab82124 is purified by an affinity chromatography in combination with FPLC.
General info
- Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Post-translational modifications
Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
1 unit equals 1 nano-gram of purified protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
- Biological function summary
AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.
- Pathways
AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.
- Associated diseases and disorders
AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
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SDS-PAGE - Recombinant Human Androgen Receptor protein (His tag) (ab82124)
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