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Recombinant Human Androgen Receptor protein (His tag) is a Human Fragment protein, in the 650 to 920 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EMSA, GSA.
Alternative names=Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, EMSA, GSA
- Biologically active
No
- Sequence
T S P T E E T T Q K L T V S H I E G Y E C Q P I F L N V L E A I E P G V V C A G H D N N Q P D S F A A L L S S L N E L G E R Q L V H V V K W A K A L P G F R N L H V D D Q M A V I Q Y S W M G L M V F A M G W R S F T N V N S R M L Y F A P D L V F N E Y R M H K S R M Y S Q C V R M R H L S Q E F G W L Q I T P Q E F L C M K A L L L F S I I P V D G L K N Q K F F D E L R M N Y I K E L D R I I A C K R K N P T S C S R R F Y Q L T K L L D S V Q P I A R E L H Q F T F D L L I K S H M V S V D F P E M M A E I I S V Q V P K I L S G K V K P I Y F H T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application EMSA | Reactivity Reacts | Dilution info - | Notes - |
Application GSA | Reactivity Reacts | Dilution info - | Notes Can be used for protein-protein and small molecules-protein interaction assays. |
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Recombinant Human Androgen Receptor protein (His tag) is a Human Fragment protein, in the 650 to 920 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EMSA, GSA.
Alternative names=Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, EMSA, GSA
- Biologically active
No
- Predicted molecular weight
33.7 kDa
- Amino acids
650 to 920
- Sequence
T S P T E E T T Q K L T V S H I E G Y E C Q P I F L N V L E A I E P G V V C A G H D N N Q P D S F A A L L S S L N E L G E R Q L V H V V K W A K A L P G F R N L H V D D Q M A V I Q Y S W M G L M V F A M G W R S F T N V N S R M L Y F A P D L V F N E Y R M H K S R M Y S Q C V R M R H L S Q E F G W L Q I T P Q E F L C M K A L L L F S I I P V D G L K N Q K F F D E L R M N Y I K E L D R I I A C K R K N P T S C S R R F Y Q L T K L L D S V Q P I A R E L H Q F T F D L L I K S H M V S V D F P E M M A E I I S V Q V P K I L S G K V K P I Y F H T Q
- Accession
- P10275-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Specifications
- Form
Liquid
- Additional notes
ab82124 is purified by an affinity chromatography in combination with FPLC.
General info
- Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Post-translational modifications
Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Unit Definition (Activity)
1 unit equals 1 nano-gram of purified protein.
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