Recombinant Human ANKRA2 protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M D T S T N L D I G A Q L I V E E C P S T Y S L T G M P D I K I E H P L D P N S E E G S A Q G V A M G M K F I L P N R F D M N V C S R F V K S L N E E D S K N I Q D Q V N S D L E V A S V L F K A E C N I H T S P S P G I Q V R H V Y T P S T T K H F S P I K Q S T T L T N K H R G N E V S T T P L L A N S L S V H Q L A A Q G E M L Y L A T R I E Q E N V I N H T D E E G F T P L M W A A A H G Q I A V V E F L L Q N G A D P Q L L G K G R E S A L S L A C S K G Y T D I V K M L L D C G V D V N E Y D W N G G T P L L Y A V H G N H V K C V K M L L E S G A D P T I E T D S G Y N S M D L A V A L G Y R S V Q Q V I E S H L L K L L Q N I K E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May regulate the interaction between the 3M complex and the histone deacetylases HDAC4 and HDAC5 (PubMed:25752541). May also regulate LRP2/megalin (By similarity).
Alternative names
ANKRA, ANKRA2, Ankyrin repeat family A protein 2, RFXANK-like protein 2
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Recombinant Human ANKRA2 protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9H9E1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M D T S T N L D I G A Q L I V E E C P S T Y S L T G M P D I K I E H P L D P N S E E G S A Q G V A M G M K F I L P N R F D M N V C S R F V K S L N E E D S K N I Q D Q V N S D L E V A S V L F K A E C N I H T S P S P G I Q V R H V Y T P S T T K H F S P I K Q S T T L T N K H R G N E V S T T P L L A N S L S V H Q L A A Q G E M L Y L A T R I E Q E N V I N H T D E E G F T P L M W A A A H G Q I A V V E F L L Q N G A D P Q L L G K G R E S A L S L A C S K G Y T D I V K M L L D C G V D V N E Y D W N G G T P L L Y A V H G N H V K C V K M L L E S G A D P T I E T D S G Y N S M D L A V A L G Y R S V Q Q V I E S H L L K L L Q N I K E
- Accession
- Q9H9E1
- Protein length
- Full Length
- Predicted molecular weight
- 36.7 kDa
- Amino acids
- 1 to 313
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab183225 was purified using conventional chromatography techniques.
General info
- Function
May regulate the interaction between the 3M complex and the histone deacetylases HDAC4 and HDAC5 (PubMed:25752541). May also regulate LRP2/megalin (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ANKRA2 also known as ankyrin repeat domain 2 is a protein with a molecular weight of about 34 kDa. It features a series of ankyrin repeat domains which are important for protein-protein interactions. ANKRA2 expresses broadly in human tissues with notable levels in the heart liver and skeletal muscle. Its structure facilitates its role in recruitment and binding of other cellular components serving as an adaptor or scaffold in several biological processes.
- Biological function summary
ANKRA2 associates with other protein structures to form complexes integral to cellular operations. It contributes to the regulation of gene expression and intracellular signaling processes. Within the nucleus ANKRA2 interacts with transcription regulatory complexes playing a role in modifying the transcription activity. This interaction allows it to contribute to the fine-tuning of gene activity affecting cell growth and differentiation.
- Pathways
ANKRA2 finds itself in connection with the Notch signaling and NF-κB pathways both important for various cellular functions. In the Notch signaling pathway ANKRA2 directly interacts with Notch receptors to modulate target gene expression. Additionally within the NF-κB pathway ANKRA2 influences the activation of NF-κB transcription factors which are vital for immune response and inflammation. Its engagement with these pathways highlights its role as a modulator in signal transduction processes.
- Associated diseases and disorders
Disturbances in ANKRA2 function can link to cancer and cardiovascular conditions. Abnormal expression or mutations of ANKRA2 may connect to disruptions in Notch signaling which have been associated with tumorigenesis. Similarly its interaction with proteins in the NF-κB pathway can influence inflammation-related diseases like atherosclerosis. Research into these connections offers potential insights for therapeutic strategies targeting ANKRA2-mediated pathways in these diseases.
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1 product image
SDS-PAGE - Recombinant Human ANKRA2 protein (ab183225)
15% SDS-PAGE analysis of ab183225 (3μg).
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