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AB93005

Recombinant Human Annexin-2/ANXA2 protein (6x His tag N-Terminus)

5

(2 Reviews)

|

(5 Publications)

Recombinant Human Annexin-2/ANXA2 protein (6x His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 0.25 EU/mL endotoxin level, suitable for SDS-PAGE, ELISA, Mass Spec, WB.

View Alternative Names

ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

2 Images
Western blot - Recombinant Human Annexin-2/ANXA2 protein (AB93005)
  • WB

Unknown

Western blot - Recombinant Human Annexin-2/ANXA2 protein (AB93005)

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (<a href='/en-us/products/primary-antibodies/annexin-2-anxa2-antibody-ab41803'>ab41803</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Annexin-2/ANXA2 protein (ab93005) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 38 kDa

true

Exposure time: 20s

SDS-PAGE - Recombinant Human Annexin-2/ANXA2 protein (AB93005)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Annexin-2/ANXA2 protein (AB93005)

ab93005 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB) and immunoblotted with anti-6x His serum (WB).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.25 EU/mL

Expression system

Escherichia coli

Tags

6x His tag N-Terminus

Applications

ELISA, SDS-PAGE, Mass Spec, WB

applications

Biologically active

No

Accession

P07355

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

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Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Proteins & Peptides

AB317875

Recombinant Human ANXA1 Protein (His-tag)

Mass Spectrometry - Recombinant Human ANXA1 Protein (His-tag) (AB317875)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab93005 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/annexin-2-anxa2-antibody-ab41803'>ab41803</a>.</p>" } } }
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Product details

This product was previously labelled as Annexin A2
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P07355","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.
Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.
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Specifications

Form

Liquid

Additional notes

Purified by 6xHis tag / Immobilized-metal affinity chromatography (IMAC).

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General info

Function

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179). Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation (By similarity). Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis (By similarity).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.

Sequence similarities

Belongs to the annexin family.

Post-translational modifications

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.. ISGylated.

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Product protocols

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179). Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation (By similarity). Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis (By similarity).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
See full target information ANXA2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18575 PubMed39048916

2024

Generation of B7-H3 isoform regulated by ANXA2/NSUN2/YBX1 axis in human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yifen Shen,Chunfang Ma,Xiangxiang Li,Xiaosong Li,Yuxiang Wu,Tao Yang,Yanping Hu,Chao Liu,Hao Shen,Pin Guo,Yihang Shen

International journal of molecular sciences 23: PubMed36293376

2022

Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Parin Klabklai,Jitrada Phetfong,Rattanawan Tangporncharoen,Chartchalerm Isarankura-Na-Ayudhya,Tulyapruek Tawonsawatruk,Aungkura Supokawej

Journal of hematology & oncology 15:141 PubMed36209111

2022

Cancer-associated fibroblast-specific lncRNA LINC01614 enhances glutamine uptake in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tongyan Liu,Chencheng Han,Panqi Fang,Zhifei Ma,Xiaoxiao Wang,Hao Chen,Siwei Wang,Fanchen Meng,Cheng Wang,Erbao Zhang,Guozhang Dong,Hongyu Zhu,Wenda Yin,Jie Wang,Xianglin Zuo,Mantang Qiu,Jinke Wang,Xu Qian,Hongbing Shen,Lin Xu,Zhibin Hu,Rong Yin

Annals of translational medicine 9:1452 PubMed34734004

2021

The important roles and molecular mechanisms of annexin A autoantibody in children with nephrotic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Ye,Yingying Zhang,Jieqiu Zhuang,Ye Bi,Hong Xu,Qian Shen,Jialu Liu,Haidong Fu,Jingjing Wang,Chunyue Feng,Xiaoxiao Tang,Fei Liu,Weizhong Gu,Fei Zhao,Jianjiang Zhang,Yuanhan Qin,Shiqiang Shang,Hongqiang Shen,Xuejun Chen,Huijun Shen,Aimin Liu,Yonghui Xia,Zhihong Lu,Qiang Shu,Jianhua Mao

eLife 9: PubMed32420875

2020

ER-luminal [Ca] regulation of InsP receptor gating mediated by an ER-luminal peripheral Ca-binding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Horia Vais,Min Wang,Karthik Mallilankaraman,Riley Payne,Chris McKennan,Jeffrey T Lock,Lynn A Spruce,Carly Fiest,Matthew Yan-Lok Chan,Ian Parker,Steven H Seeholzer,J Kevin Foskett,Don-On Daniel Mak
View all publications
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