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AB92968

Recombinant Human Annexin-6/ANXA6 protein (6x His)

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Recombinant Human Annexin-6/ANXA6 protein (6x His) is a Human Full Length protein, in the 1 to 673 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA, Mass Spec, WB.

View Alternative Names

ANX6, ANXA6, Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70, CPB-II

2 Images
Western blot - Recombinant Human Annexin-6/ANXA6 protein (AB92968)
  • WB

Unknown

Western blot - Recombinant Human Annexin-6/ANXA6 protein (AB92968)

All lanes:

Anti-Annexin VI antibody (<a href='/en-us/products/unavailable/annexin-6anxa6-antibody-ab52221'>ab52221</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human Annexin-6/ANXA6 protein (ab92968) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 1min

SDS-PAGE - Recombinant Human Annexin-6/ANXA6 protein (AB92968)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Annexin-6/ANXA6 protein (AB92968)

10% SDS-PAGE showing ab92968
Lane 1 MWM
Lane 2 Stained with Coomassie Blue
Lane 3 Immunobloting with anti-6xHis serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag

Applications

ELISA, Mass Spec, WB, SDS-PAGE

applications

Biologically active

No

Accession

P08133

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 0.68% Imidazole Constituents: 2.9% Sodium chloride, 0.316% Tris HCl, 0.0078% 2-Mercaptoethanol

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB199422

Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

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  • 0
Proteins & Peptides

AB123173

Recombinant Human Annexin-7/ANXA7 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Annexin-7/ANXA7 protein (His tag N-Terminus) (AB123173)

  • 0
  • 0
Proteins & Peptides

AB269138

Recombinant human Wwp2 protein (Active)

Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)

  • 0
  • 0
Proteins & Peptides

AB269130

Recombinant human VCP protein (Active)

Functional Studies - Recombinant human VCP protein (Active) (AB269130)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p><a href='/en-us/products/unavailable/annexin-6anxa6-antibody-ab52221'>ab52221</a> can be used as a WB positive control in conjunction with ab92968.</p>" } } }
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Product details

This product was previously labelled as Annexin VI
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":673,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P08133","tags":[{"tag":"6x His","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-6 also known as ANXA6 is a member of the annexin family of proteins. It has a mass of approximately 68 kDa and is expressed in various tissues with substantial presence in muscle and liver. This protein acts mechanically by binding to membranes in a calcium-dependent manner playing a role in membrane-associated processes such as endocytosis and exocytosis. Annexin-6 can also interact with cytoskeletal elements influencing cell structure and stability.
Biological function summary

Annexin-6 functions as a versatile scaffold that organizes multiprotein complexes. It modulates membrane trafficking processes and participates in signaling pathways within the cell. This protein can influence intracellular calcium homeostasis by interacting with calcium channels and transporters making it an integral part of cellular signaling and communication.

Pathways

Annexin-6 plays a role in the MAPK and PI3K/Akt signaling pathways. It associates with proteins such as Ras and Protein Kinase C impacting cell proliferation survival and differentiation. These interactions highlight the importance of annexin-6 in cellular responses to external stimuli and help regulate various physiological processes in the body.

Annexin-6 has been linked to cardiac disorders and cancer. In the context of cardiac health it interacts with proteins like phospholamban and calmodulin to influence heart muscle function and calcium regulation. In cancer annexin-6's involvement in the Akt signaling pathway can affect tumor growth and metastasis often interacting with other proteins like EGFR and HER2. This makes it a significant protein of interest in disease research and potential therapeutic targeting.
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Specifications

Form

Liquid

Additional notes

Purified by 6xHis tag / Immobilized-metal affinity chromatography (IMAC).

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General info

Function

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

Sequence similarities

Belongs to the annexin family.

Post-translational modifications

Phosphorylated in response to growth factor stimulation.

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Product protocols

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Target data

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
See full target information ANXA6
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Product promise

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