Recombinant Human Annexin V/ANXA5 protein is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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- Diabetes, metabolic syndrome and obesity : targets and therapy 14:2335-23472021[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Yuan Shen,Miao Wang,Zhihua Liu,Shurong Wang,Ying XiePubMed 34079312
- Biomedical optics express 7:2641-92016Lowering photosensitizer doses and increasing fluences induce apoptosis in tumor bearing mice.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab89493 can be used as a WB positive control in conjunction with ab30941. |
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Target data
Function
The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha
Recommended products
Recombinant Human Annexin V/ANXA5 protein is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P08758-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- Accession
- P08758
- Protein length
- Full Length
- Amino acids
- 1 to 320
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab89493 is purified using conventional chromatography techniques.
General info
- Function
The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the annexin family.
- Post-translational modifications
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Annexin V
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
- Biological function summary
Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.
- Pathways
Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.
- Associated diseases and disorders
Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
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2 product images
SDS-PAGE - Recombinant Human Annexin V/ANXA5 protein (ab89493)
SDS-PAGE analysis of Recombinant Human Annexon V protein (ab89493), under reducing conditions. Proteins visualized by coomassie blue stain.
Lane 1: Molecular Weight Standards
Lane 2: 3 μg Recombinant Human Annexon V protein
Western blot - Recombinant Human Annexin V/ANXA5 protein (ab89493)
All lanes: Anti-Annexin V antibody (ab30941) at 1 µg/mL
All lanes: Western blot - Recombinant Human Annexin V/ANXA5 protein (ab89493) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20s
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