Recombinant Human Annexin V/ANXA5 protein (His tag) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A Q V L R G T V T D F P G F D E R A D A E T L R K A M K G L G T D E E S I L T L L T S R S N A Q R Q E I S A A F K T L F G R D L L D D L K S E L T G K F E K L I V A L M K P S R L Y D A Y E L K H A L K G A G T N E K V L T E I I A S R T P E E L R A I K Q V Y E E E Y G S S L E D D V V G D T S G Y Y Q R M L V V L L Q A N R D P D A G I D E A Q V E Q D A Q A L F Q A G E L K W G T D E E K F I T I F G T R S V S H L R K V F D K Y M T I S G F Q I E E T I D R E T S G N L E Q L L L A V V K S I R S I P A Y L A E T L Y Y A M K G A G T D D H T L I R V M V S R S E I D L F N I R K E F R K N F A T S L Y S M I K G D T S G D Y K K A L L L L C G E D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Alternative names
Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Recommended products
Recombinant Human Annexin V/ANXA5 protein (His tag) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P08758-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS
Sequence info
Amino acid sequence
M A Q V L R G T V T D F P G F D E R A D A E T L R K A M K G L G T D E E S I L T L L T S R S N A Q R Q E I S A A F K T L F G R D L L D D L K S E L T G K F E K L I V A L M K P S R L Y D A Y E L K H A L K G A G T N E K V L T E I I A S R T P E E L R A I K Q V Y E E E Y G S S L E D D V V G D T S G Y Y Q R M L V V L L Q A N R D P D A G I D E A Q V E Q D A Q A L F Q A G E L K W G T D E E K F I T I F G T R S V S H L R K V F D K Y M T I S G F Q I E E T I D R E T S G N L E Q L L L A V V K S I R S I P A Y L A E T L Y Y A M K G A G T D D H T L I R V M V S R S E I D L F N I R K E F R K N F A T S L Y S M I K G D T S G D Y K K A L L L L C G E D D
- Accession
- Protein length
Full Length
- Predicted molecular weight
36.8 kDa
- Amino acids
1 to 320
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
ab157342 is lyophilized from 0.22 µm filtered solution.
General info
- Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
- Sequence similarities
Belongs to the annexin family.
- Post-translational modifications
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was previously labelled as Annexin V.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
- Biological function summary
Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.
- Pathways
Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.
- Associated diseases and disorders
Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
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1 product image
SDS-PAGE - Recombinant Human Annexin V/ANXA5 protein (His tag) (ab157342)
SDS-PAGE analysis of reduced ab157342 stained overnight with Coomassie Blue. The protein migrates as 35 kDa under reducing conditions.
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