Recombinant human Annexin V/ANXA5 protein (PE) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >=89% purity and suitable for SDS-PAGE, HPLC, Flow Cyt.
>=89% HPLC
Escherichia coli
Tag free
SDS-PAGE, HPLC, Flow Cyt
Yes
PE
M A Q V L R G T V T D F P G F D E R A D A E T L R K A M K G L G T D E E S I L T L L T S R S N A Q R Q E I S A A F K T L F G R D L L D D L K S E L T G K F E K L I V A L M K P S R L Y D A Y E L K H A L K G A G T N E K V L T E I I A S R T P E E L R A I K Q V Y E E E Y G S S L E D D V V G D T S G Y Y Q R M L V V L L Q A N R D P D A G I D E A Q V E Q D A Q A L F Q A G E L K W G T D E E K F I T I F G T R S V S H L R K V F D K Y M T I S G F Q I E E T I D R E T S G N L E Q L L L A V V K S I R S I P A Y L A E T L Y Y A M K G A G T D D H T L I R V M V S R S E I D L F N I R K E F R K N F A T S L Y S M I K G D T S G D Y K K A L L L L C G E D D
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
ANX5, ENX2, PP4, ANXA5, ANX5, ENX2, PP4, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PP4, PAP-I, VAC-alpha
Recombinant human Annexin V/ANXA5 protein (PE) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >=89% purity and suitable for SDS-PAGE, HPLC, Flow Cyt.
>=89% HPLC
Escherichia coli
Tag free
SDS-PAGE, HPLC, Flow Cyt
Yes
Binds to phosphatidylserine (PS). Exhibits anti-phospholipase activity.
PE
Ex: 480;565nm, Em: 578nm
No
Human
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 0.61% Tris, 0.58% Sodium chloride
M A Q V L R G T V T D F P G F D E R A D A E T L R K A M K G L G T D E E S I L T L L T S R S N A Q R Q E I S A A F K T L F G R D L L D D L K S E L T G K F E K L I V A L M K P S R L Y D A Y E L K H A L K G A G T N E K V L T E I I A S R T P E E L R A I K Q V Y E E E Y G S S L E D D V V G D T S G Y Y Q R M L V V L L Q A N R D P D A G I D E A Q V E Q D A Q A L F Q A G E L K W G T D E E K F I T I F G T R S V S H L R K V F D K Y M T I S G F Q I E E T I D R E T S G N L E Q L L L A V V K S I R S I P A Y L A E T L Y Y A M K G A G T D D H T L I R V M V S R S E I D L F N I R K E F R K N F A T S L Y S M I K G D T S G D Y K K A L L L L C G E D D
Full Length
35.8 kDa
1 to 320
Recombinant
Liquid
ab157016 was determined to be >= 89% pure by SDS-PAGE and HPLC.
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Belongs to the annexin family.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Blue Ice
+4°C
+4°C
Do Not Freeze, Store in the dark
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab157016 can be used for detection of apoptotic cells by flow cytometry. It is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable secondary antibody. This product was previously labelled as Annexin V
This supplementary information is collated from multiple sources and compiled automatically.
Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.
Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.
Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
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