Recombinant Human ANXA1 Protein (His-tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for HPLC, MS.
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Key facts
- Purity
- >=95% HPLC
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
M A M V S E F L K Q A W F I E N E E Q E Y V Q T V K S S K G G P G S A V S P Y P T F N P S S D V A A L H K A I M V K G V D E A T I I D I L T K R N N A Q R Q Q I K A A Y L Q E T G K P L D E T L K K A L T G H L E E V V L A L L K T P A Q F D A D E L R A A M K G L G T D E D T L I E I L A S R T N K E I R D I N R V Y R E E L K R D L A K D I T S D T S G D F R N A L L S L A K G D R S E D F G V N E D L A D S D A R A L Y E A G E R R K G T D V N V F N T I L T T R S Y P Q L R R V F Q K Y T K Y S K H D M N K V L D L E L K G D I E K C L T A I V K C A T S K P A F F A E K L H Q A M K G V G T R H K A L I R I M V S R S E I D M N D I K A F Y Q K M Y G I S L C Q A I L D E T K G D Y E K I L V A L C G G N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).
Alternative names
ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35
Recommended products
Recombinant Human ANXA1 Protein (His-tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for HPLC, MS.
Key facts
- Purity
- >=95% HPLC
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P04083-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.4862% Sodium chloride, 0.3002% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A M V S E F L K Q A W F I E N E E Q E Y V Q T V K S S K G G P G S A V S P Y P T F N P S S D V A A L H K A I M V K G V D E A T I I D I L T K R N N A Q R Q Q I K A A Y L Q E T G K P L D E T L K K A L T G H L E E V V L A L L K T P A Q F D A D E L R A A M K G L G T D E D T L I E I L A S R T N K E I R D I N R V Y R E E L K R D L A K D I T S D T S G D F R N A L L S L A K G D R S E D F G V N E D L A D S D A R A L Y E A G E R R K G T D V N V F N T I L T T R S Y P Q L R R V F Q K Y T K Y S K H D M N K V L D L E L K G D I E K C L T A I V K C A T S K P A F F A E K L H Q A M K G V G T R H K A L I R I M V S R S E I D M N D I K A F Y Q K M Y G I S L C Q A I L D E T K G D Y E K I L V A L C G G N
- Accession
- P04083
- Protein length
- Full Length
- Predicted molecular weight
- 39.78 kDa
- Actual molecular weight
- 39.65 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
- Sequence similarities
Belongs to the annexin family.
- Post-translational modifications
Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:15485879, PubMed:2457390). Phosphorylated in response to EGF treatment (PubMed:2532504).
- Subcellular localisation
- Nucleus, Endosome membrane, Early endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.
- Biological function summary
Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.
- Pathways
Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.
- Associated diseases and disorders
Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.
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2 product images
Mass Spectrometry - Recombinant Human ANXA1 Protein (His-tag) (ab317875)
Mass determination by ESI-TOF. Predicted MW is 39780 Da (+/- 10 Da by ESI-TOF). Observed MW is 39646.88 Da.
HPLC - Recombinant Human ANXA1 Protein (His-tag) (ab317875)
HPLC analysis of ab317875
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