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AB171698

Recombinant Human AP1AR/Gadkin protein

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Recombinant Human AP1AR/Gadkin protein is a Human Full Length protein, in the 1 to 302 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C4orf16, PRO0971, AP1AR, AP-1 complex-associated regulatory protein, 2c18, Adaptor-related protein complex 1-associated regulatory protein, Gamma-1-adaptin brefeldin A resistance protein, Gamma-A1-adaptin and kinesin interactor, GBAR, Gamma-BAR, Gadkin

1 Images
SDS-PAGE - Recombinant Human AP1AR/Gadkin protein (AB171698)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AP1AR/Gadkin protein (AB171698)

15% SDS-PAGE analysis of ab171698 (3 µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q63HQ0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as AP1AR
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGNCCWTQCFGLLRKEAGRLQRVGGGGGSKYFRTCSRGEHLTIEFENLVESDEGESPGSSHRPLTEEEIVDLRERHYDSIAEKQKDLDKKIQKELALQEEKLRLEEEALYAAQREAARAAKQRKLLEQERQRIVQQYHPSNNGEYQSSGPEDDFESCLRNMKSQYEVFRSSRLSSDATVLTPNTESSCDLMTKTKSTSGNDDSTSLDLEWEDEEGMNRMLPMRERSKTEEDILRAALKYSNKKTGSNPTSASDDSNGLEWENDFVSAEMDDNGNSEYSGFVNPVLELSDSGIRHSDTDQQTR","proteinLength":"Full Length","predictedMolecularWeight":"36.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":302,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q63HQ0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP1AR also known as Gadkin is an adapter protein involved in intracellular trafficking. It has a molecular weight of approximately 45 kDa. This protein predominantly expresses in the brain and various neurons playing an important role in assembling clathrin-coated vesicles. AP1AR works by interacting with other coat proteins and sorting cargo molecules assisting in vesicle formation and release.
Biological function summary

AP1AR functions as part of clathrin-mediated endocytosis an essential process for recycling synaptic vesicles and neurotransmitter release. In neurons AP1AR partners with other vesicle-associated proteins to execute accurate sorting of membrane proteins. Its involvement aides in nerve signal transmission contributing to proper synaptic function. This function makes AP1AR integral to maintaining neuron health and efficiency.

Pathways

AP1AR operates within the clathrin-mediated endocytosis and protein trafficking pathways. It interacts with the AP-1 complex important for vesicular transport especially in neuronal cells. AP1AR affects the dynamics of these pathways by maintaining the balance of synaptic vesicle formation and retrieval important for neuronal communication and health. Its relationship with other proteins like AP-2 and dynamin ensures the seamless execution of these trafficking steps.

AP1AR shows connections to neurological conditions such as Alzheimer’s disease and Parkinson’s disease where synaptic dysfunction occurs. Disruption in AP1AR-related pathways can contribute to the pathology of these diseases impacting neurological health. Additionally its connection to proteins like tau in Alzheimer's and alpha-synuclein in Parkinson’s highlights AP1AR's role in neurodegenerative disease progression. Understanding its function and interactions might lead to therapeutic insights.
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Specifications

Form

Liquid

Additional notes

ab171698 is purified using conventional chromatography techniques

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General info

Function

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.

Post-translational modifications

Palmitoylated.

Subcellular localisation

Late endosome

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Product protocols

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Target data

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.
See full target information AP1AR
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