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AB127858

Recombinant Human AP4E1 protein (His-DHFR)

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Recombinant Human AP4E1 protein (His-DHFR) is a Human Fragment protein, in the 951 to 1136 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

AP-4 complex subunit epsilon-1, AP-4 adaptor complex subunit epsilon, Adaptor-related protein complex 4 subunit epsilon-1, Epsilon subunit of AP-4, Epsilon-adaptin, AP4E1

Key facts

Purity

>95%

Expression system

Escherichia coli

Tags

His-DHFR tag

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UPM8

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB316003

Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (AB316003)

  • 0
  • 0
Primary Antibodies

AB318190

Anti-AP4E1 antibody [EPR28049-87] - BSA and Azide free

Recombinant|RabMAb

Western blot - Anti-AP4E1 antibody [EPR28049-87] - BSA and Azide free (AB318190)

  • 0
  • 0
Primary Antibodies

AB122124

Anti-SLCO4A1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO4A1 antibody (AB122124)

  • 0
  • 0
Primary Antibodies

AB98861

Anti-PBA1 antibody

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1136,"aminoAcidStart":951,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UPM8","tags":[{"tag":"His-DHFR","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP4E1 also known as Adaptor Related Protein Complex 4 Epsilon 1 Subunit functions as a component of the adaptor protein complex 4 (AP-4). It has a mass of approximately 110 kDa. AP4E1 plays a role in the recruitment of clathrin-coated vesicles and is found mainly in the trans-Golgi network. The protein is expressed in several tissues with notable presence in the central nervous system and various epithelial cells.
Biological function summary

AP4E1 facilitates the sorting of cargo proteins at the trans-Golgi network contributing to intracellular trafficking. It forms part of the AP-4 complex that includes other subunits like AP4S1 AP4B1 and AP4M1. This complex binds to sorting signals on the cytoplasmic tails of membrane proteins directing them to their appropriate intracellular destinations.

Pathways

AP4E1 participates in protein sorting mechanisms that influence the traffic of lysosomal enzymes and other cargo proteins. It interacts with SNX27 a protein that links endosomal sorting to signal transduction pathways. AP4E1 has relations with pathways involving endocytosis and membrane trafficking particularly those that regulate cellular homeostasis and signaling.

Altered function or expression of AP4E1 associates with neurological disorders such as hereditary spastic paraplegia. Mutations in this protein can lead to disruptions in neural pathways similar to the effects seen with related proteins like AP4B1. Studies suggest connections to other genetic conditions involving cognitive impairment highlighting the protein's importance in proper neuronal function.
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Specifications

Form

Lyophilized

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General info

Function

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed : 10066790, PubMed : 10436028). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (Probable).

Sequence similarities

Belongs to the adaptor complexes large subunit family.

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Product protocols

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Target data

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed : 10066790, PubMed : 10436028). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (Probable).
See full target information AP4E1
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