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AB198064

Recombinant Human APAF1 protein (His tag N-Terminus + DDDDK tag N-Terminus)

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(1 Publication)

Recombinant Human APAF1 protein (His tag N-Terminus + DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 1237 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA0413, APAF1, Apoptotic protease-activating factor 1, APAF-1

1 Images
SDS-PAGE - Recombinant Human APAF1 protein (His tag N-Terminus + DDDDK tag N-Terminus) (AB198064)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human APAF1 protein (His tag N-Terminus + DDDDK tag N-Terminus) (AB198064)

4-20% SDS-PAGE analysis of 2.6 μg ab198064 with Coomassie staining.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O14727

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB234436

Anti-APAF1 antibody [EPR21112-102]

KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-APAF1 antibody [EPR21112-102] (AB234436)

  • 0
  • 0
Primary Antibodies

AB133504

Anti-Cytochrome C antibody [EPR1327]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (AB133504)

  • 5
  • 2
Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHHHHHHDYKDDDDKDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSETQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE","proteinLength":"Full Length","predictedMolecularWeight":"143 kDa","actualMolecularWeight":null,"aminoAcidEnd":1237,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O14727","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APAF1 also known as apoptotic protease activating factor 1 plays a mechanical role in apoptosis by forming a complex with other proteins to trigger cell death. The APAF1 protein has a molecular weight of approximately 130 kDa. It is expressed in many tissues but occurs highly in the brain and heart. APAF1 interacts with cytochrome c and procaspase-9 to promote the activation of the initiator caspase cascade.
Biological function summary

APAF1 functions as a central component of the apoptosome a multi-protein complex important for programmed cell death. This process is essential for normal development and cellular homeostasis. In the apoptosome APAF1 recruits and activates procaspase-9 leading to the activation of caspase-3. This cascade assures that cells die in a controlled manner preventing damage to surrounding tissues.

Pathways

APAF1 serves an important function in the intrinsic pathway of apoptosis. It mediates cytochrome c release from mitochondria a step essential for apoptosis initiation. Another important pathway involving APAF1 is the apoptotic signaling pathway. Proteins such as Bcl-2 family members and p53 influence APAF1's role in these pathways by regulating cytochrome c release and apoptosome assembly.

APAF1 is notably associated with neurodegenerative diseases and certain cancers. Dysregulation of APAF1 can lead to undesirable apoptosis contributing to neurodegenerative conditions like Parkinson’s disease. Conversely reduced APAF1 activity can result in tumor cell survival impacting cancer progression. Altered expression of APAF1 affects its interaction with proteins like Bcl-2 which can inhibit apoptosis and promote cancer cell growth.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

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Product protocols

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Target data

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
See full target information APAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2201889 PubMed35975461

2022

APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Wang,Chen Chen,Xiao Xu,Chuanjun Shu,Changchang Cao,Zhangding Wang,Yao Fu,Lei Xu,Kaiyue Xu,Jiawen Xu,Anliang Xia,Bo Wang,Guifang Xu,Xiaoping Zou,Ruibao Su,Wei Kang,Yuanchao Xue,Ran Mo,Beicheng Sun,Shouyu Wang
View all publications
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Product promise

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