Recombinant Human Apc1 protein is a Human Fragment protein, in the 1841 to 1944 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
N S E F L P V V K C T I D N T L D Q W L Q V G G D M C V H A Y L S G Q P L E E S Q L S M L A C F L V Y H S V P A P Q H L P P I G L E G S T S F A E L L F K F K Q L K M P V R A L L R L A P L L L G N P Q P M V M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
Alternative names
TSG24, ANAPC1, Anaphase-promoting complex subunit 1, APC1, Cyclosome subunit 1, Mitotic checkpoint regulator, Testis-specific gene 24 protein
Recommended products
Recombinant Human Apc1 protein is a Human Fragment protein, in the 1841 to 1944 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9H1A4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- N S E F L P V V K C T I D N T L D Q W L Q V G G D M C V H A Y L S G Q P L E E S Q L S M L A C F L V Y H S V P A P Q H L P P I G L E G S T S F A E L L F K F K Q L K M P V R A L L R L A P L L L G N P Q P M V M
- Accession
- Q9H1A4
- Protein length
- Fragment
- Amino acids
- 1841 to 1944
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
- Sequence similarities
Belongs to the APC1 family.
- Post-translational modifications
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Apc1 protein also known as Anaphase Promoting Complex subunit 1 is a core component of the Anaphase Promoting Complex/Cyclosome (APC/C). With a molecular mass of approximately 215 kDa Apc1 functions within the cell cycle particularly in regulating progression. It is expressed in the nucleus of eukaryotic cells and plays a significant role in governing the ubiquitination and degradation of cell cycle regulators which helps ensure orderly cell division.
- Biological function summary
The Anaphase Promoting Complex subunit Apc1 is vital in executing the degradation of securin and cyclin B during the cell cycle. This activity is integral to the transition from metaphase to anaphase during mitosis. Apc1 participates as a structural core within the APC/C complex—a multi-subunit E3 ubiquitin ligase complex. The APC/C complex controls protein ligation marking them for proteasomal degradation and thereby contributing to accurate mitosis and meiosis.
- Pathways
Apc1 operates within the cell cycle and ubiquitin-proteasome system pathways. It plays a pivotal part in regulating the metaphase-anaphase transition and exits from mitosis. The protein associates with key cell cycle proteins such as CDC20 and CDH1 which serve as activators for the APC/C complex modulating its activity across different phases of the cell cycle.
- Associated diseases and disorders
The misregulation of Apc1 associates with cancer and certain chromosomal instability syndromes. Aberrant APC/C activity can result in uncontrolled cell proliferation observed in various cancer types including colorectal cancer wherein Apc1 may connect to other proteins like p53 a critical tumor suppressor mutated in many cancers. Altered Apc1 function and consequent cell cycle errors implicate genetic instability which can underlie chromosomal instability syndromes.
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1 product image
SDS-PAGE - Recombinant Human Apc1 protein (ab163855)
ab163855 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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