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Recombinant Human APG5L/ATG5 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Alternative names=Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M T D D K D V L R D V W F G R I P T C F T L Y Q D E I T E R E A E P Y Y L L L P R V S Y L T L V T D K V K K H F Q K V M R Q E D I S E I W F E Y E G T P L K W H Y P I G L L F D L L A S S S A L P W N I T V H F K S F P E K D L L H C P S K D A I E A H F M S C M K E A D A L K H K S Q V I N E M Q K K D H K Q L W M G L Q N D R F D Q F W A I N R K L M E Y P A E E N G F R Y I P F R I Y Q T T T E R P F I Q K L F R P V A A D G Q L H T L G D L L K E V C P S A I D P E D G E K K N Q V M I H G I E P M L E T P L Q W L S E H L S Y P D N F L H I S I I P Q P T D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human APG5L/ATG5 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Alternative names=Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Predicted molecular weight
34.6 kDa
- Amino acids
1 to 275
- Sequence
M G S S H H H H H H S S G L V P R G S H M T D D K D V L R D V W F G R I P T C F T L Y Q D E I T E R E A E P Y Y L L L P R V S Y L T L V T D K V K K H F Q K V M R Q E D I S E I W F E Y E G T P L K W H Y P I G L L F D L L A S S S A L P W N I T V H F K S F P E K D L L H C P S K D A I E A H F M S C M K E A D A L K H K S Q V I N E M Q K K D H K Q L W M G L Q N D R F D Q F W A I N R K L M E Y P A E E N G F R Y I P F R I Y Q T T T E R P F I Q K L F R P V A A D G Q L H T L G D L L K E V C P S A I D P E D G E K K N Q V M I H G I E P M L E T P L Q W L S E H L S Y P D N F L H I S I I P Q P T D
- Accession
- Q9H1Y0-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
ab103787 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
- Sequence similarities
Belongs to the ATG5 family.
- Post-translational modifications
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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