Recombinant Human APIP protein is a Human Full Length protein, in the 1 to 242 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S G C D A R E G D C C S R R C G A Q D K E H P R Y L I P E L C K Q F Y H L G W V T G T G G G I S L K H G D E I Y I A P S G V Q K E R I Q P E D M F V C D I N E K D I S G P S P S K K L K K S Q C T P L F M N A Y T M R G A G A V I H T H S K A A V M A T L L F P G R E F K I T H Q E M I K G I K K C T S G G Y Y R Y D D M L V V P I I E N T P E E K D L K D R M A H A M N E Y P D S C A V L V R R H G V Y V W G E T W E K A K T M C E C Y D Y L F D I A V S M K K V G L D P S Q L P V G E N G I V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
Alternative names
CGI-29, APIP, Methylthioribulose-1-phosphate dehydratase, MTRu-1-P dehydratase, APAF1-interacting protein, hAPIP
Recommended products
Recombinant Human APIP protein is a Human Full Length protein, in the 1 to 242 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96GX9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S G C D A R E G D C C S R R C G A Q D K E H P R Y L I P E L C K Q F Y H L G W V T G T G G G I S L K H G D E I Y I A P S G V Q K E R I Q P E D M F V C D I N E K D I S G P S P S K K L K K S Q C T P L F M N A Y T M R G A G A V I H T H S K A A V M A T L L F P G R E F K I T H Q E M I K G I K K C T S G G Y Y R Y D D M L V V P I I E N T P E E K D L K D R M A H A M N E Y P D S C A V L V R R H G V Y V W G E T W E K A K T M C E C Y D Y L F D I A V S M K K V G L D P S Q L P V G E N G I V
- Accession
- Q96GX9
- Protein length
- Full Length
- Predicted molecular weight
- 29.7 kDa
- Amino acids
- 1 to 242
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab128440 is purified using conventional chromatography techniques
General info
- Function
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
- Sequence similarities
Belongs to the aldolase class II family. MtnB subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
APIP also known as APAF1-interacting protein weighs around 26 kDa and serves as an anti-apoptotic factor. This protein is widely expressed in various tissues including the liver brain and heart. Mechanically APIP regulates apoptosis by interacting with APAF1 which prevents the activation of the apoptosome. The inhibition of apoptosis helps cells survive under stress conditions.
- Biological function summary
APIP plays a significant role in modulating the cellular mechanism of apoptosis. As part of the apoptosis inhibition complex it interacts with APAF1 to control programmed cell death. Additionally APIP contributes to cell survival during oxidative stress by modulating the methionine salvage pathway and maintaining cellular redox balance.
- Pathways
APIP integrates into essential cellular systems such as the apoptosis and methionine salvage pathways. By interacting with APAF1 in the apoptotic pathway APIP directly influences the intrinsic pathway of apoptosis. At the same time it connects with enzymes involved in methionine metabolism supporting cellular survival mechanisms through antioxidant defense.
- Associated diseases and disorders
APIP shows a connection to cancer and ischemic injury. In cancer APIP confers resistance to apoptosis promoting tumor survival even under cellular stress. Its interaction with APAF1 plays an important role in this mechanism. In ischemic conditions APIP involvement in the methionine salvage pathway helps prevent oxidative damage by supporting cellular redox balance potentially linking it to proteins involved in ischemic protection.
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1 product image
SDS-PAGE - Recombinant Human APIP protein (ab128440)
15% SDS-PAGE showing ab128440 (3 µg).
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