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AB276373

Recombinant human Apo-H protein (His tag)

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Recombinant human Apo-H protein (His tag) is a Human Full Length protein, in the 1 to 345 aa range, expressed in HEK 293 cells, with >96%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

B2G1, APOH, Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Beta-2-glycoprotein I, Apo-H, B2GPI, Beta(2)GPI

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Functional Studies - Recombinant human Apo-H protein (His tag) (AB276373)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Apo-H protein (His tag) (AB276373)

Measured by its binding ability in a functional ELISA.

Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated human LDLR-his.

The EC50 of biotinylated human LDLR-his is 0.35 μg/mL.

Functional Studies - Recombinant human Apo-H protein (His tag) (AB276373)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Apo-H protein (His tag) (AB276373)

Measured by its binding ability in a functional ELISA.

Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated mouse LDLR-his.

The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.

SDS-PAGE - Recombinant human Apo-H protein (His tag) (AB276373)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Apo-H protein (His tag) (AB276373)

SDS-PAGE analysis of ab276373

Key facts

Purity

>96% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 μg/mL.

Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated mouse LDLR-his, The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.

Accession

P02749

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MISPVLILFSSFLCHVAIAGRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPC","proteinLength":"Full Length","predictedMolecularWeight":"37.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":345,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P02749","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein H (Apo-H) also known as β2-glycoprotein I functions mechanically as a plasma protein. It has a molecular weight of approximately 50 kDa and it is mostly expressed in the liver. Apo-H plays a role in the body's immune and coagulation systems binding to anionic surfaces such as phospholipids and preventing blood coagulation. Its ability to interact with negatively charged molecules makes it important in the modulation of the coagulation cascade helping maintain balance between bleeding and clotting.
Biological function summary

Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.

Pathways

Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.

Apo-H associates with antiphospholipid syndrome and cardiovascular diseases. These conditions arise from its role in coagulation and immune response where disruptions can lead to increased risk of thrombosis. The protein's interaction with other apolipoproteins such as Apo-A1 highlights possible implications in lipid metabolism disorders which in turn can exacerbate cardiovascular risk. Understanding these associations provides insights into potential therapeutic targets for treating these diseases.
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Specifications

Form

Lyophilized

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General info

Function

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Post-translational modifications

N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.

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Product protocols

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Target data

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
See full target information APOH
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