Recombinant Human Apo-M protein is a Human Fragment protein, in the 23 to 188 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M C P E H S Q L T T L G V D G K E F P E V H L G Q W Y F I A G A A P T K E E L A T F D P V D N I V F N M A A G S A P M Q L H L R A T I R M K D G L C V P R K W I Y H L T E G S T D L R T E G R P D M K T E L F S S S C P G G I M L N E T G Q G Y Q R F L L Y N R S P H P P E K C V E E F K S L T S C L D S K A F L L T P R N Q E A C E L S N N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Alternative names
G3A, NG20, HSPC336, APOM, Apolipoprotein M, Apo-M, ApoM, Protein G3a
Recommended products
Recombinant Human Apo-M protein is a Human Fragment protein, in the 23 to 188 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O95445-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M C P E H S Q L T T L G V D G K E F P E V H L G Q W Y F I A G A A P T K E E L A T F D P V D N I V F N M A A G S A P M Q L H L R A T I R M K D G L C V P R K W I Y H L T E G S T D L R T E G R P D M K T E L F S S S C P G G I M L N E T G Q G Y Q R F L L Y N R S P H P P E K C V E E F K S L T S C L D S K A F L L T P R N Q E A C E L S N N
- Accession
- O95445
- Protein length
- Fragment
- Predicted molecular weight
- 20.9 kDa
- Amino acids
- 23 to 188
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab93935 was purified using conventional chromatography techniques.
General info
- Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
- Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein M (Apo-M) also known as protein G3a is a 25 kDa member of the lipocalin family which is secreted primarily by liver and kidney cells. Apo-M is a component of high-density lipoprotein (HDL) where it plays a significant role in lipid metabolism. It serves as a binding partner for lipophilic molecules such as sphingosine-1-phosphate (S1P) impacting the stability and functionality of HDL particles. Researchers use anti-Apo antibodies to investigate the expression localization and function of Apo-M in various conditions.
- Biological function summary
Apo-M influences several critical processes. It binds with S1P which is involved in endothelial barrier function vascular development and immune cell trafficking. Apo-M is also important for maintaining the inverse relationship between cholesterol levels and cardiovascular risk. It functions within HDL complexes affecting cholesterol efflux and anti-inflammatory responses. Apart from HDL Apo-M interacts with other Apo proteins like apoA-I and apoA-II serving as a regulatory component within these lipoprotein complexes.
- Pathways
Apo-M integrates into lipid metabolism and signal transduction pathways. It participates in the S1P signaling pathway which is important for maintaining vascular integrity and immune function. Apo-M's interactions within this pathway influence the activity of other proteins such as the S1P receptors which play a critical role in mediating its biological activities. Additionally Apo-M interfaces with cholesterol efflux pathways interacting with the ATP-binding cassette transporters particularly ABCA1 that facilitate cholesterol removal from peripheral tissues.
- Associated diseases and disorders
Apo-M has associations with atherosclerosis and metabolic syndrome. Dysregulation of Apo-M levels can contribute to the development of atherosclerotic plaques as it affects HDL's protective roles against oxidation and inflammation. Alterations in Apo-M levels may also influence metabolic syndrome components given its function in lipid metabolism and glucose homeostasis. In these contexts changes in Apo-M can affect proteins like apoE which are involved in lipid transport and cardiovascular risk modulation.
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SDS-PAGE - Recombinant Human Apo-M protein (ab93935)
15% SDS-PAGE analysis of 3μg ab93935
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