Recombinant Human Apo-M Protein Standard is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
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Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, sELISA
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Alternative names
Apolipoprotein M, Apo-M, ApoM, Protein G3a, HSPC336, NG20, G3A, APOM
Recommended products
Recombinant Human Apo-M Protein Standard is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
Alternative names
Apolipoprotein M, Apo-M, ApoM, Protein G3a, HSPC336, NG20, G3A, APOM
Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, sELISA
- Biologically active
No
- Accession
- O95445-1
- Animal free
Yes
- Species
Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Predicted molecular weight
21.6 kDa
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
- Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Recombinant Human Apo-M Protein Standard (ab322694)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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