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AB116768

Recombinant Human APOBEC1 protein

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Recombinant Human APOBEC1 protein is a Human Fragment protein, in the 137 to 236 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

C->U-editing enzyme APOBEC-1, Apolipoprotein B mRNA-editing enzyme catalytic subunit 1, HEPR, mRNA(cytosine(6666)) deaminase 1, APO1, APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, APOBEC1

1 Images
SDS-PAGE - Recombinant Human APOBEC1 protein (AB116768)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human APOBEC1 protein (AB116768)

12.5% SDS-PAGE showing ab116768 at approximately 36.63kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P41238

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Sequence info

[{"sequence":"SGVTIQIMRASEYYHCWRNFVNYPPGDEAHWPQYPPLWMMLYALELHCIILSLPPCLKISRRWQNHLTFFRLHLQNCHYQTIPPHILLATGLIHPSVAWR","proteinLength":"Fragment","predictedMolecularWeight":"36.63 kDa","actualMolecularWeight":null,"aminoAcidEnd":236,"aminoAcidStart":137,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P41238","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC1 also known as Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 1 is an enzyme responsible for the deamination of cytidine to uridine in mRNA. This editing capability focuses mainly on apolipoprotein B (apoB) mRNA affecting lipid metabolism significantly. The APOBEC1 protein weighs approximately 27 kDa. It expresses mainly in the small intestine and liver where lipid processing is an essential biological function.
Biological function summary

APOBEC1 plays an important role in lipid homeostasis by editing apoB mRNA transcripts leading to the production of a shorter form of the apoB protein. This protein acts as a critical component in lipid transport and metabolism. APOBEC1 functions within a multisubunit complex that includes other proteins like A1CF (APOBEC1 Complementation Factor). This protein complex ensures efficient mRNA editing necessary for optimal lipid regulation.

Pathways

The APOBEC1 enzyme is part of key metabolic routes notably lipid metabolism and lipid-transport routes. It contributes to the post-transcriptional modification of apoB mRNA influencing pathways like lipoprotein assembly. Through these pathways related proteins such as microsomal triglyceride transfer protein (MTP) work alongside APOBEC1 to facilitate lipid processing. MTP assists in lipoprotein biogenesis highlighting a collaborative function in lipid management.

APOBEC1 has associations with conditions such as atherosclerosis and hepatic steatosis. Aberrant expression or function of APOBEC1 can lead to improper lipid metabolism directly linking to the development of atherosclerosis due to the accumulation of cholesterol-rich lipoproteins. Furthermore hepatic steatosis or fatty liver disease may arise when lipid homeostasis deteriorates. In these conditions APOBEC1 along with proteins like apoB becomes central to understanding disease mechanisms and potential therapeutic interventions.
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Specifications

Form

Liquid

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General info

Function

Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs (PubMed : 30844405). Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA (PubMed : 24916387). Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA (PubMed : 11727199). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs (PubMed : 30844405). Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA (PubMed : 24916387). Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA (PubMed : 11727199). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).
See full target information APOBEC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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