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AB160558

Recombinant Human APOBEC3B protein (GST tag N-Terminus)

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Recombinant Human APOBEC3B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 251 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

1 Images
SDS-PAGE - Recombinant Human APOBEC3B protein (GST tag N-Terminus) (AB160558)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human APOBEC3B protein (GST tag N-Terminus) (AB160558)

ab160558 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9UH17

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB184990

Anti-APOBEC3B antibody [EPR18138]

RabMAb|Recombinant

Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990)

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Proteins & Peptides

AB235615

Recombinant Human PHO1 protein (Tagged)

SDS-PAGE - Recombinant Human PHO1 protein (Tagged) (AB235615)

  • 0
  • 0
Primary Antibodies

AB302926

Anti-APOBEC3G/A3G antibody [EPR25404-59]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

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  • 0
Proteins & Peptides

AB159787

Recombinant Human UQCRFS1/RISP protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human UQCRFS1/RISP protein (GST tag N-Terminus) (AB159787)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFEPQYHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVKIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRLRIFSVAFTAAMRSCASWTWFLLCSWTRPRSTGSLGSSPGAPASPGAVPGKCVRSFRRTHT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":251,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9UH17","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3B also known as Apolipoprotein B mRNA editing enzyme catalytic subunit 3B is a protein that plays a role in DNA cytosine deamination. It selectively deaminates cytosine bases in DNA to uracil resulting in mutations. The protein has a molecular mass of approximately 46 kDa. APOBEC3B expression occurs primarily in the liver and testes but it can also be detected in other tissues.
Biological function summary

APOBEC3B functions as part of the innate immune defense by restricting viral replication through inducing hypermutation in viral genomes. It does not form a stable complex but can act in conjunction with other APOBEC3 family members. APOBEC3B activity is critical in the context of host-virus interactions especially in the defense against retroviruses and other viruses.

Pathways

Scientists classify APOBEC3B within the viral restriction and DNA damage response pathways. In these pathways the protein works alongside other DNA repair proteins such as AID (Activation-Induced cytidine Deaminase). APOBEC3B participates in editing events that provide insights into virus-host co-evolution and resilience against viral infections.

Researchers have linked APOBEC3B to cancer development due to its ability to induce genetic mutations. Its mutagenic activity connects it with a variety of cancers including breast cancer and head and neck cancer. Through these diseases the protein shows functional connections to other DNA mutators and repair proteins that it affects or interacts with during tumorigenesis.
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Specifications

Form

Liquid

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General info

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.
See full target information APOBEC3B
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