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AB132965

Recombinant Human APOBEC3G/A3G protein (GST tag N-Terminus)

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Recombinant Human APOBEC3G/A3G protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 384 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

MDS019, APOBEC3G, DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase, APOBEC-related protein 9, CEM-15, Deoxycytidine deaminase, APOBEC-related protein, ARCD, ARP-9, CEM15, A3G

1 Images
SDS-PAGE - Recombinant Human APOBEC3G/A3G protein (GST tag N-Terminus) (AB132965)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human APOBEC3G/A3G protein (GST tag N-Terminus) (AB132965)

12.5% SDS-PAGE analysis of ab132965 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q9HC16

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

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Recombinant Human APOBEC1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human APOBEC1 protein (AB116768)

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Primary Antibodies

AB302926

Anti-APOBEC3G/A3G antibody [EPR25404-59]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

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Proteins & Peptides

AB160558

Recombinant Human APOBEC3B protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human APOBEC3B protein (AB160558)

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Proteins & Peptides

AB165460

Recombinant Human APOBEC3D protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human APOBEC3D protein (GST tag N-Terminus) (AB165460)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as APOBEC3G.
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Sequence info

[{"sequence":"MKPHFRNTVERMYRDTFSYNFYNRPILSRRNTVWLCYEVKTKGPSRPPLDAKIFRGQVYSELKYHPEMRFFHWFSKWRKLHRDQEYEVTWYISWSPCTKCTRDMATFLAEDPKVTLTIFVARLYYFWDPDYQEALRSLCQKRDGPRATMKIMNYDEFQHCWSKFVYSQRELFEPWNNLPKYYILLHIMLGEILRHSMDPPTFTFNFNNEPWVRGRHETYLCYEVERMHNDTWVLLNQRRGFLCNQAPHKHGFLEGRHAELCFLDVIPFWKLDLDQDYRVTCFTSWSPCFSCAQEMAKFISKNKHVSLCIFTARIYDDQGRCQEGLRTLAEAGAKISIMTYSEFKHCWDTFVDHQGCPFQPWDGLDEHSQDLSGRLRAILQNQEN","proteinLength":"Full Length","predictedMolecularWeight":"72.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":384,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9HC16","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3G also known as A3G is an enzyme that belongs to the APOBEC family of cytidine deaminases. It has a molecular mass of approximately 46 kDa. This protein is expressed in various tissues including immune cells like lymphocytes and macrophages. A3G plays a mechanical role in catalyzing the deamination of cytidine to uridine on single-stranded DNA which impacts the genetic material of retroviruses.
Biological function summary

APOBEC3G acts as a host defense mechanism against retroviral infections particularly HIV-1. It incorporates into budding virions where it exerts its antiviral effect. A3G functions within the APOBEC protein family complex that participates in innate immunity. This antiviral activity helps to inhibit viral replication by inducing lethal mutations in the viral genome.

Pathways

APOBEC3G is vital in the HIV-1 life cycle disruption pathway. It interacts with other cellular factors such as the viral protein Vif which counters APOBEC3G’s activity by targeting it for proteasomal degradation. The protein is also involved in the regulation of gene expression pathways linked to innate immune responses.

APOBEC3G is associated with HIV-1 infection. Elevated levels of APOBEC3G can lead to the hypermutation of viral DNA potentially reducing viral loads in host cells. The protein’s interaction with HIV-1 Vif protein is critical as Vif facilitates the degradation of A3G allowing the virus to escape its antiviral activity. Additionally researchers investigate a possible connection between A3G and cancer due to its mutation-inducing capability in DNA.
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Specifications

Form

Liquid

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General info

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms (PubMed : 12808465, PubMed : 16527742, PubMed : 17121840, PubMed : 18288108, PubMed : 18849968, PubMed : 19153609, PubMed : 21123384, PubMed : 22791714, PubMed : 25542899). Exhibits potent antiviral activity against Vif-deficient HIV-1 (PubMed : 12167863, PubMed : 12859895, PubMed : 14557625, PubMed : 20219927, PubMed : 21835787, PubMed : 22807680, PubMed : 22915799, PubMed : 23097438, PubMed : 23152537, PubMed : 31397674). After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA (PubMed : 12808465, PubMed : 12808466, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300, PubMed : 22807680). The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells (PubMed : 12808465, PubMed : 12808466, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300). Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA (PubMed : 12808465, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300). Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) (PubMed : 15031497, PubMed : 16378963, PubMed : 18448976, PubMed : 19458006, PubMed : 20335265). May inhibit the mobility of LTR and non-LTR retrotransposons (PubMed : 16527742).

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Post-translational modifications

(Microbial infection) Following infection by HIV-1, ubiquitinated by a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, leading to its degradation (PubMed:14528300, PubMed:14528301, PubMed:19887642, PubMed:22190037, PubMed:23300442, PubMed:31253590, PubMed:36754086, PubMed:37419875). Deubiquitinated by USP49; leading to stabilization (PubMed:31397674).. Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms (PubMed : 12808465, PubMed : 16527742, PubMed : 17121840, PubMed : 18288108, PubMed : 18849968, PubMed : 19153609, PubMed : 21123384, PubMed : 22791714, PubMed : 25542899). Exhibits potent antiviral activity against Vif-deficient HIV-1 (PubMed : 12167863, PubMed : 12859895, PubMed : 14557625, PubMed : 20219927, PubMed : 21835787, PubMed : 22807680, PubMed : 22915799, PubMed : 23097438, PubMed : 23152537, PubMed : 31397674). After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA (PubMed : 12808465, PubMed : 12808466, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300, PubMed : 22807680). The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells (PubMed : 12808465, PubMed : 12808466, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300). Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA (PubMed : 12808465, PubMed : 12809610, PubMed : 12970355, PubMed : 14528300). Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) (PubMed : 15031497, PubMed : 16378963, PubMed : 18448976, PubMed : 19458006, PubMed : 20335265). May inhibit the mobility of LTR and non-LTR retrotransposons (PubMed : 16527742).
See full target information APOBEC3G
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