Recombinant Human APOF protein is a Human Full Length protein, in the 37 to 327 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
T S Y G K Q T N V L M H F P L S L E S Q T P S S D P L S C Q F L H P K S L P G F S H M A P L P K F L V S L A L R N D A L E E A G C Q A D V W A L Q L Q L Y R Q G G V N A T Q V L I Q H L R G L Q K G R S T E R N V S V E A L A S A L Q L L A R E Q Q S T G R V G R S L P T E D C E N E K E Q A V H N V V Q L L P G V G T F Y N L G T A L Y Y A T Q N C L G K A R E R G R D G A I D L G Y D L L M T M A G M S G G P M G L A I S A A L K P A L R S G V Q Q L I Q Y Y Q D Q K D A N I S Q P E T T K E G L R A I S D V S D L E E T T T L A S F I S E V V S S A P Y W G W A I I K S Y D L D P G A G S L E I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein. |
Target data
Function
Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Alternative names
Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF
Recommended products
Recombinant Human APOF protein is a Human Full Length protein, in the 37 to 327 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q13790-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- T S Y G K Q T N V L M H F P L S L E S Q T P S S D P L S C Q F L H P K S L P G F S H M A P L P K F L V S L A L R N D A L E E A G C Q A D V W A L Q L Q L Y R Q G G V N A T Q V L I Q H L R G L Q K G R S T E R N V S V E A L A S A L Q L L A R E Q Q S T G R V G R S L P T E D C E N E K E Q A V H N V V Q L L P G V G T F Y N L G T A L Y Y A T Q N C L G K A R E R G R D G A I D L G Y D L L M T M A G M S G G P M G L A I S A A L K P A L R S G V Q Q L I Q Y Y Q D Q K D A N I S Q P E T T K E G L R A I S D V S D L E E T T T L A S F I S E V V S S A P Y W G W A I I K S Y D L D P G A G S L E I
- Accession
- Q13790
- Protein length
- Full Length
- Predicted molecular weight
- 58.01 kDa
- Amino acids
- 37 to 327
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
- Sequence similarities
Belongs to the apolipoprotein F family.
- Post-translational modifications
O-glycosylated with core 1 or possibly core 8 glycans.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Apolipoprotein F.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The APOF protein also known as Apolipoprotein F serves a significant role in lipid metabolism. This protein has a molecular weight of approximately 29 kDa. APOF is expressed mainly in the liver and is found in the blood plasma. It is part of the lipoprotein system where it associates with lipoproteins as it circulates within the bloodstream.
- Biological function summary
The APOF protein helps regulate lipid transport and cholesterol ester uptake. It is involved in the modulation of the plasma lipoprotein levels important for maintaining lipid homeostasis. APOF does not work independently but interacts with other lipoproteins in the plasma to regulate lipid transfer processes. Its control over cholesterol homeostasis hints at its involvement in complex lipoprotein metabolic pathways.
- Pathways
APOF plays a part in the reverse cholesterol transport pathways. This pathway is essential for the maintenance of cholesterol balance in the body. APOF affects the function of very low-density lipoproteins (VLDL) and is related to other proteins like apolipoprotein A1 which also contribute to cholesterol transport and lipoprotein metabolism.
- Associated diseases and disorders
An imbalance or alteration in APOF levels is linked to hyperlipoproteinemia and cardiovascular diseases. These conditions are associated with cholesterol and triglyceride metabolism where APOF and related proteins like low-density lipoprotein (LDL) play significant roles. The modulation of APOF activity presents a potential target for therapeutic strategies in managing lipid-related disorders.
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1 product image
SDS-PAGE - Recombinant Human APOF protein (ab116968)
12.5% SDS-PAGE showing ab116968 at approximately 58.01kDa stained with Coomassie Blue.
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